The interfacial pH of acidic degradable polymeric biomaterials and its effects on osteoblast behavior

• Published in: Scientific reports Vol. 7; no. 1; pp. 6794 - 12
• Main Authors: Ruan, Changshun, Hu, Nan, Ma, Yufei, Li, Yuxiao, Liu, Juan, Zhang, Xinzhou, Pan, Haobo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28-07-2017; Nature Publishing Group; Nature Portfolio
• Subjects: 13/107; 639/301/54/2295; 639/301/54/990; Animals; Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Biodegradability; Biodegradable materials; Biodegradable Plastics - chemistry; Biodegradable Plastics - pharmacology; Biodegradation; Biomaterials; Biomedical materials; Bone growth; Cell Differentiation; Cells, Cultured; Contact angle; Degradation products; Humanities and Social Sciences; Hydrogen-Ion Concentration; Investigations; Metabolites; Morphology; multidisciplinary; Osteoblasts; Osteoblasts - cytology; Osteoblasts - drug effects; pH effects; Piperazine; Piperazine - chemistry; Polyesters; Polyesters - chemistry; Polymers; Polyurethane; Polyurethanes - chemistry; Rats; Rats, Sprague-Dawley; Regeneration; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-06354-1

A weak alkaline environment is established to facilitate the growth of osteoblasts. Unfortunately, this is inconsistent with the application of biodegradable polymer in bone regeneration, as the degradation products are usually acidic. In this study, the variation of the interfacial pH of poly (D, L-lactide) and piperazine-based polyurethane ureas (P-PUUs), as the representations of acidic degradable materials, and the behavior of osteoblasts on these substrates with tunable interfacial pH were investigated
in vitro
. These results revealed that the release of degraded products caused a rapid decrease in the interfacial pH, and this could be relieved by the introduction of alkaline segments. On the contrary, when culturing with osteoblasts, the variation of the interfacial pH revealed an upward tendency, indicating that cell could construct the microenvironment by secreting cellular metabolites to satisfy its own survival. In addition, the behavior of osteoblasts on substrates exhibited that P-PUUs with the most PP units were better for cell growth and osteogenic differentiation of cells. This is due to the hydrophilic surface and the moderate N% in P-PUUs, key factors in the promotion of the early stages of cellular responses, and the interfacial pH contributing to the enhanced effect on osteogenic differentiation.