Transcriptome analysis of nicotine-exposed cells from the brainstem of neonate spontaneously hypertensive and Wistar Kyoto rats

• Published in: The pharmacogenomics journal Vol. 10; no. 2; pp. 134 - 160
• Main Authors: Ferrari, M F R, Reis, E M, Matsumoto, J P P, Fior-Chadi, D R
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01-04-2010; Nature Publishing Group
• Subjects: Animals; Animals, Newborn; Biomedical and Life Sciences; Biomedicine; Brain stem; Brain Stem - drug effects; Brain Stem - metabolism; Cell interactions; DNA microarrays; Gene Expression; Gene Expression - drug effects; Gene Expression Profiling; Genetic aspects; Genomes; Health aspects; Human Genetics; Hypertension; Hypertension - genetics; Intracellular signalling; Nicotine; Nicotine - pharmacology; Oligonucleotide Array Sequence Analysis; Oncology; original-article; Pharmacotherapy; Physiological aspects; Psychopharmacology; Rats; Rats, Inbred SHR - metabolism; Rats, Inbred WKY - metabolism; Transcriptomes; Brazil; microarray; nicotine; primary cell culture; brainstem; hypertension; gene expression
• ISSN: 1470-269X; 1473-1150
• DOI: 10.1038/tpj.2009.42

In this study, the effects of nicotine on global gene expression of cultured cells from the brainstem of spontaneously hypertensive rat (SHR) and normotensive Wistar Kyoto (WKY) rats were evaluated using whole-genome oligoarrays. We found that nicotine may act differentially on the gene expression profiles of SHR and WKY. The influence of strain was present in 321 genes that were differentially expressed in SHR as compared with WKY brainstem cells independently of the nicotine treatment. A total of 146 genes had their expression altered in both strains after nicotine exposure. Interaction between nicotine treatment and the strain was observed to affect the expression of 229 genes that participate in cellular pathways related to neurotransmitter secretion, intracellular trafficking and cell communication, and are possibly involved in the phenotypic differentiation between SHR and WKY rats, including hypertension. Further characterization of their function in hypertension development is warranted.