A bioactivated in vivo assembly nanotechnology fabricated NIR probe for small pancreatic tumor intraoperative imaging

• Published in: Nature communications Vol. 13; no. 1; pp. 418 - 13
• Main Authors: Ren, Han, Zeng, Xiang-Zhong, Zhao, Xiao-Xiao, Hou, Da-yong, Yao, Haodong, Yaseen, Muhammad, Zhao, Lina, Xu, Wan-hai, Wang, Hao, Li, Li-Li
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20-01-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/61/2298; 631/61/54/152; 631/67/2321; Accumulation; Alanine Transaminase - metabolism; Alkaline Phosphatase - metabolism; Amino Acid Sequence; Animals; Aspartate Aminotransferases - metabolism; Assembly; Biological activity; Blood circulation; Cell Line, Tumor; Female; Fluorescence; Fluorescent Dyes - chemistry; Fluorescent Dyes - toxicity; Fluorescent indicators; Humanities and Social Sciences; Humans; Image enhancement; Intraoperative Care; Liver - enzymology; Medical imaging; Mice; Mice, Inbred BALB C; Mice, Nude; Molecular Dynamics Simulation; multidisciplinary; Nanofibers; Nanofibers - chemistry; Nanotechnology; Optical Imaging; Pancreatic cancer; Pancreatic Neoplasms - diagnostic imaging; Pancreatic Neoplasms - surgery; Peptides - chemistry; Probes; Protein Conformation; Science; Science (multidisciplinary); Selectivity; Surgery; Tissue Distribution; Toxicity Tests, Acute; Tumors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-27932-y

Real-time imaging of the tumour boundary is important during surgery to ensure that sufficient tumour tissue has been removed. However, the current fluorescence probes for bioimaging suffer from poor tumour specificity and narrow application of the imaging window used. Here, we report a bioactivated in vivo assembly (BIVA) nanotechnology, demonstrating a general optical probe with enhanced tumour accumulation and prolonged imaging window. The BIVA probe exhibits active targeting and assembly induced retention effect, which improves selectivity to tumours. The surface specific nanofiber assembly on the tumour surface increases the accumulation of probe at the boundary of the tumor. The blood circulation time of the BIVA probe is prolonged by 110 min compared to idocyanine green. The assembly induced metabolic stability broaden the difference between the tumor and background, obtaining a delayed imaging window between 8–96 h with better signal-to-background contrast (>9 folds). The fabricated BIVA probe permits precise imaging of small sized (<2 mm) orthotopic pancreatic tumors in vivo. The high specificity and sensitivity of the BIVA probe may further benefit the intraoperative imaging in a clinical setting.
Fluorescence probes for detecting tumours during surgery can suffer from poor accumulation and short imaging windows. Here, the author develop fluorescence probes with multiple motifs that permit enhanced circulation times, tumour targeting and use the probes to image pancreatic cancer in mice