Amalaki rasayana, a traditional Indian drug enhances cardiac mitochondrial and contractile functions and improves cardiac function in rats with hypertrophy

• Published in: Scientific reports Vol. 7; no. 1; pp. 8588 - 17
• Main Authors: Kumar, Vikas, Aneesh, kumar A., Kshemada, K., Ajith, Kumar G. S., Binil, Raj S. S., Deora, Neha, Sanjay, G., Jaleel, A., Muraleedharan, T. S., Anandan, E. M., Mony, R. S, Valiathan, M. S., Santhosh, Kumar T. R., Kartha, C. C
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17-08-2017; Nature Publishing Group; Nature Portfolio
• Subjects: 14; 631/443/7; 64; 692/4019/592/1540; 82/29; 82/51; 82/58; Aging; Aging - metabolism; Animals; Antioxidants; Aorta; Aorta - pathology; Aorta - physiopathology; Ca2+-transporting ATPase; Cardiomegaly - drug therapy; Cardiomegaly - genetics; Cardiomegaly - physiopathology; Cell Death - drug effects; Constriction, Pathologic; Energy Metabolism - drug effects; Fatigue; Fibrosis; Gene Expression Regulation - drug effects; Heart diseases; High-performance liquid chromatography; Histology; Humanities and Social Sciences; Hypertrophy; Liquid chromatography; Male; Medicine, Traditional; Mitochondria; Mitochondria, Heart - drug effects; Mitochondria, Heart - metabolism; Models, Biological; multidisciplinary; Muscle contraction; Myocardial Contraction - drug effects; NF-κB protein; Oxidative phosphorylation; Phagocytosis; Phosphorylation; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Pressure; Rats, Wistar; Rodents; Science; Science (multidisciplinary); Tricarboxylic acid cycle; Ventricle
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-09225-x

We evaluated the cardioprotective effect of Amalaki Rasayana (AR), a rejuvenating Ayurvedic drug prepared from
Phyllanthus
emblica
fruits in the reversal of remodeling changes in pressure overload left ventricular cardiac hypertrophy (LVH) and age-associated cardiac dysfunction in male Wistar rats. Six groups (aging groups) of 3 months old animals were given either AR or ghee and honey (GH) orally; seventh group was untreated. Ascending aorta was constricted using titanium clips in 3 months old rats (N = 24; AC groups) and after 6 months, AR or GH was given for further 12 months to two groups; one group was untreated. Histology, gene and protein expression analysis were done in heart tissues. Chemical composition of AR was analyzed by HPLC, HPTLC and LC-MS. AR intake improved (
P
 < 0.05) cardiac function in aging rats and decreased LVH (
P
 < 0.05) in AC rats as well as increased (
P
 < 0.05) fatigue time in treadmill exercise in both groups. In heart tissues of AR administered rats of both the groups, SERCA2, CaM, Myh11, antioxidant, autophagy, oxidative phosphorylation and TCA cycle proteins were up regulated. ADRB1/2 and pCREB expression were increased; pAMPK, NF-kB were decreased. AR has thus a beneficial effect on myocardial energetics, muscle contractile function and exercise tolerance capacity.