Searching for DNA in museum specimens: a comparison of sources in a mammal species

• Published in: Molecular ecology resources Vol. 10; no. 3; pp. 502 - 507
• Main Authors: CASAS-MARCE, M, REVILLA, E, GODOY, J.A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-05-2010; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: Amplification; Bones; Deoxyribonucleic acid; Diaphysis; DNA; Genetic markers; Hides; historical samples; Lynx; microsatellite DNA; Microsatellites; Minimum cost; Mitochondria; mitochondrial DNA; museum specimens; Museums; real-time qPCR
• ISSN: 1755-0998; 1755-098X; 1755-0998
• DOI: 10.1111/j.1755-0998.2009.02784.x

The number of genetic studies that use preserved specimens as sources of DNA has been steadily increasing during the last few years. Therefore, selecting the sources that are more likely to provide a suitable amount of DNA of enough quality to be amplified and at the minimum cost to the original specimen is an important step for future research. We have compared different types of tissue (hides vs. bones) from museum specimens of Iberian lynx and multiple alternative sources within each type (skin, footpad, footpad powder, claw, diaphysis, maxilloturbinal bone, mastoid process and canine) for DNA yield and probability of amplification of both mitochondrial and nuclear targets. Our results show that bone samples yield more and better DNA than hides, particularly from sources from skull, such as mastoid process and canines. However, claws offer an amplification success as high as bone sources, which makes them the preferred DNA source when no skeletal pieces have been preserved. Most importantly, these recommended sources can be sampled incurring minimal damage to the specimens while amplifying at a high success rate for both mitochondrial and microsatellite markers.