Changes in peripheral blood oxidative stress markers and hepatic gene expression related to oxidative stress in Holstein cows with and without subacute ruminal acidosis during the periparturient period

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 10; pp. 1529 - 1536
• Main Authors: TSUCHIYA, Yoshiyuki, OZAI, Reiko, SUGINO, Toshihisa, KAWASHIMA, Kenji, KUSHIBIKI, Shiro, KIM, Yo-Han, SATO, Shigeru
• Format: Journal Article
• Language: English
• Published: Tokyo JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Acidosis; Antioxidants; Aspartate transaminase; Energy balance; Gene expression; Glutathione peroxidase; Glutathione transferase; hepatic gene expression; Holstein cow; Internal Medicine; Liver; Malondialdehyde; Metabolites; Metallothionein; Oxidation; Oxidative stress; Parturition; periparturient period; Peripheral blood; Postpartum; subacute ruminal acidosis; Superoxide dismutase; Transaminase
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.20-0426

We investigated changes in peripheral blood metabolites, oxidative stress markers (malondialdehyde, potential antioxidant capacity, and glutathione peroxidase [GPX]), and hepatic gene expression related to oxidative stress in Holstein cows with and without subacute ruminal acidosis (SARA) during the periparturient period. Eighteen multiparous Holstein cows were categorized into SARA (n=9) or non-SARA (n=9) groups depending on whether they developed SARA; reticulo-ruminal pH was <5.6 for more than 3 hr per day, during the 2 weeks after parturition. Blood and liver tissue samples were collected 3 weeks prepartum and 2 and 6 weeks postpartum, with an additional blood sample collected 0 and 4 weeks postpartum. Blood aspartate transaminase (AST) and nonesterified fatty acid (NEFA) increased significantly (P<0.05) after parturition in both groups. GPX activity decreased gradually after parturition in the SARA group. In the SARA group, gene expression of GPX 1 and microsomal glutathione S-transferase 3 (MGST3) decreased significantly (P<0.05), and expression of metallothionein 2A increased significantly (P<0.05) after parturition in the SARA group. Superoxide dismutase 1 and MGST3 decreased significantly (P<0.05) 2 weeks postpartum in the non-SARA group. Gene expression related to oxidative stress was negatively correlated with AST, NEFA and total ketone body levels. Therefore, the hepatic gene expression related to oxidative stress might change associated with a negative energy balance, and might relate the high oxidative stress in the SARA group during periparturient period.