Lacticaseibacillus rhamnosus MG4706 Suppresses Periodontitis in Osteoclasts, Inflammation-Inducing Cells, and Ligature-Induced Rats

• Published in: Nutrients Vol. 14; no. 22; p. 4869
• Main Authors: Kim, Seonyoung, Lee, Ji Yeon, Park, Jeong-Yong, Kim, YongGyeong, Kang, Chang-Ho
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 17-11-2022; MDPI
• Subjects: Acid phosphatase; Acid phosphatase (tartrate-resistant); Acid resistance; Alveolar bone; Animals; anti-inflammatory; anti-osteoclastogenesis; Bacteria; Bone loss; c-Fos protein; Cathepsin K; Cytokines; Disease; Experiments; Fos gene; Gelatinase B; Gene expression; Global health; Gum disease; Humans; Inflammation; Inflammation - metabolism; Inflammatory diseases; Interleukin 6; Interleukin-6 - genetics; Lactic acid; Lactic acid bacteria; Lacticaseibacillus rhamnosus; Lipopolysaccharides; Lymphocytes; Matrix metalloproteinase; Matrix Metalloproteinase 8; Matrix Metalloproteinase 9 - genetics; Matrix metalloproteinases; Metalloproteinase; Neutrophil collagenase; NF-AT protein; Nitric oxide; Nitric-oxide synthase; Osteoclastogenesis; Osteoclasts; Osteoprotegerin; Periodontitis; Periodontitis - drug therapy; Periodontitis - microbiology; Probiotics; Public health; RANKL/OPG system; Rats; Statistical analysis; Tartrate-Resistant Acid Phosphatase; Teeth; Transcription factors; United States > US; anti-inflammatory; RANKL/OPG system; anti-osteoclastogenesis; lactic acid bacteria; periodontitis
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu14224869

Periodontitis is a chronic inflammatory disease characterized by tooth loss due to inflammation and the loss of alveolar bone. Periodontitis is closely related to various systemic diseases and is emerging as a global health problem. In this study, we investigated the anti-inflammatory effect of lactic acid bacteria (LAB) in vitro on Porphyromonas gingivalis (P. gingivalis) LPS-activated RAW264.7 and human gingival fibroblasts-1 (HGF-1) cells and the anti-osteoclastogenic effect of LAB on RANKL-induced RAW264.7 cells. All LAB strains (Lacticaseibacillus rhamnosus MG4706, MG4709, and MG4711) inhibited nitric oxide (NO)/inducible nitric oxide synthase (iNOS) in P. gingivalis LPS-activated RAW264.7 cells and pro-inflammatory cytokines (IL-1β and IL-6) and matrix metalloproteinase (MMP-8 and MMP-9) in HGF-1 cells. In addition, LAB treatment inhibited osteoclastogenesis by reducing tartrate-resistant acid phosphatase (TRAP) activity and cathepsin K (CtsK) through the downregulation of nuclear factor of activated T cells cytoplasmic 1 (NFATc1) and c-fos gene expression in RANKL-induced RAW264.7 cells. Administration of MG4706 alleviated alveolar bone loss indices and reduced the gene expression of IL-1β, IL-6, MMP-8, MMP-9, and RANKL/OPG ratio in gingival tissue. In conclusion, L. rhamnosus MG4706 has the potential to alleviate periodontitis.