Protective effect of rutin, a flavonol glycoside, on the carcinogen-induced DNA damage and repair enzymes in rats

• Published in: Cancer letters Vol. 109; no. 1; pp. 185 - 191
• Main Authors: Webster, R.P., Gawde, M.D., Bhattacharya, R.K.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 03-12-1996; Elsevier
• Subjects: Aflatoxin B 1; Aflatoxin B1 - toxicity; Animals; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Carcinogens - toxicity; Chemical agents; Dimethylnitrosamine - toxicity; DNA - drug effects; DNA damage; DNA Damage - drug effects; DNA Ligases - metabolism; DNA repair enzymes; Enzyme Induction - drug effects; Male; Medical sciences; N-Nitrosodimethylamine; Protective role; Rats; Rats, Wistar; Rutin; Rutin - pharmacology; Tumors; N-Nitrosodimethylamine; Rutin; Aflatoxin B 1; DNA damage; Protective role; DNA repair enzymes; Rat; Enzyme; Toxicity; Transferases; Rodentia; DNA ligase (ATP); Phosphoric ester ligases; Anticarcinogen; Prevention; Carcinogen; Nucleotidyltransferases; Vertebrata; Mammalia; Single strand break; Ligases; Animal; DNA; Aflatoxin B1; Glycoside; Flavone(3-hydroxy); Repair; DNA-directed DNA polymerase
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(96)04443-6

Administration of hepatocarcinogens aflatoxin B
1 and
N-nitrosodimethylamine to rats caused single-strand breaks in nuclear DNA. Inclusion in the diet of rutin, a naturally occurring phenolic flavonoid glycoside, significantly reduced the appearance of such breaks. The protection against DNA damage was found to be reduction in the induction of repair enzymes polymerase, DNA polymerase β and DNA ligase. Even associated with poly(ADP-ribose) a marginal dose of rutin was effective in this regard. Since DNA damage and inefficient repair are expected to initiate the process of carcinogenesis, modulation by rutin of these parameters emphasizes the protective role of this flavonoid against carcinogenesis induced by chemical carcinogens.