Combining dose and injection volume for good performance of a specific radiopharmaceutical for sentinel node detection

• Published in: Nuclear medicine and biology Vol. 39; no. 1; pp. 145 - 153
• Main Authors: Núñez, Eutimio Gustavo Fernández, de Oliveira, Erica Aparecida, da Silva, Natanael Gomes, de Oliveira Filho, Renato Santos
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 2012; Elsevier
• Subjects: Animal models; Animals; Biological and medical sciences; Contrast media. Radiopharmaceuticals; Cysteine - administration & dosage; Cysteine - analogs & derivatives; Cysteine - pharmacokinetics; Dextrans - administration & dosage; Dextrans - pharmacokinetics; Dosage; Female; Injected volume; Lymph nodes; Lymph Nodes - diagnostic imaging; Lymph Nodes - metabolism; Mannose - administration & dosage; Mannose - pharmacokinetics; Medical sciences; Nuclear medicine; Organotechnetium Compounds - administration & dosage; Organotechnetium Compounds - pharmacokinetics; Pharmaceuticals; Pharmacology. Drug treatments; Radioisotopes; Radiology; Radionuclide Imaging; Radiopharmaceuticals - administration & dosage; Radiopharmaceuticals - pharmacokinetics; Rats; Rats, Wistar; Sentinel Lymph Node Biopsy - methods; Sentinel lymph node detection; Technetium-99m; Tissue Distribution; Tissue-specific radiopharmaceutical; Tissue-specific radiopharmaceutical; Sentinel lymph node detection; Dosage; Technetium-99m; Injected volume; Performance evaluation; Nuclear medicine; Sentinel lymph node; Technetium 99mTc; Diagnosis; Radioisotopic product; Biomedical engineering
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/j.nucmedbio.2011.07.004

Abstract Introduction The aim of this work was to quantify the effects of injection volume at different technetium-99m specific radiotracer doses on its lymphatic movement in animal model. Procedures Effects of injection volume (50, 100 μl) at different doses (0.05, 0.135, 0.22 nmol) on popliteal node (PN) detection were studied in rats. The radiotracer under study was99m Technetium-cysteine-mannose-dextran conjugate (30 kDa). Results At 0.05 nmol dose, higher PN uptake was observed at 50 μl injection volume (2.6 fold increase). Conversely, at 0.135 nmol dose, an increase of radiotracer retention in PN was achieved at 100 μl volume, 78% higher than 50 μl. However, at 0.22 nmol dose, the injection volume changes did not influence on the PN uptake. Considering as suitable radiotracer performance: high PN uptake and extraction, better combinations were 0.05 nmol/50 μl, 0.135 nmol/100 μl, 0.22/50 μl. Conclusion Suitable performances could be reached by proper combinations of dose, injection volume and concentration for a specific radiotracer used in sentinel lymph node detection.