Expression and bioactivity analysis of recombinant beta-CPP dimer

Beta-casein phosphopeptide (beta-CPP) is a bioactive peptide that carries different minerals, especially calcium. To investigate more effects of beta-CPP, eukaryotic expression vector of beta-CPP dimer was constructed and transfected into Chinese hamster ovary (CHO) cells. After selection, the cell...

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Bibliographic Details
Published in:	Journal of dairy science Vol. 87; no. 10; p. 3198
Main Authors:	Jiang, Y J, Li, Q Z, Yan, H B, Geng, L J
Format:	Journal Article
Language:	English
Published:	United States 01-10-2004
Subjects:	Animals Apoptosis - drug effects Calcium - metabolism Caseins - genetics Caseins - pharmacology Cell Division - drug effects CHO Cells Cricetinae Dimerization Gene Expression Genetic Vectors HT29 Cells Humans Male Mice Mice, Inbred BALB C Neoplasms - pathology PC12 Cells Phosphopeptides - genetics Phosphopeptides - pharmacology Rabbits Rats Recombinant Proteins - pharmacology Spermatozoa - drug effects Spermatozoa - metabolism Spleen - cytology Transfection
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Summary:	Beta-casein phosphopeptide (beta-CPP) is a bioactive peptide that carries different minerals, especially calcium. To investigate more effects of beta-CPP, eukaryotic expression vector of beta-CPP dimer was constructed and transfected into Chinese hamster ovary (CHO) cells. After selection, the cell lines stably expressing beta-CPP dimer were obtained, and the recombinant product was identified and purified. Activity assay of recombinant protein indicated that the recombinant beta-CPP dimer could improve Ca(2+) uptake of sperm, stimulate the proliferation of spleen cells, and induce apoptosis of some malignant tumor cells.
ISSN:	0022-0302
DOI:	10.3168/jds.S0022-0302(04)73455-4