Dynamic colonisation by different Pneumocystis jirovecii genotypes in cystic fibrosis patients

• Published in: Clinical microbiology and infection Vol. 13; no. 10; pp. 1008 - 1011
• Main Authors: Montes-Cano, M.A., de la Horra, C., Dapena, F.J., Mateos, I., Friaza, V., Respaldiza, N., Muñoz-Lobato, F., Medrano, F.J., Calderon, E.J., Varela, J.M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier Ltd 01-10-2007; Blackwell Publishing Ltd; Blackwell
• Subjects: Adolescent; Adult; Biological and medical sciences; Carriage; Carrier State - epidemiology; Carrier State - microbiology; Child; Child, Preschool; cystic fibrosis; Cystic Fibrosis - complications; epidemiology; Errors of metabolism; Female; Genotype; genotypes; Humans; Infant; Male; Medical sciences; Metabolic diseases; Miscellaneous hereditary metabolic disorders; Mitochondria - genetics; molecular analysis; Molecular Epidemiology; Pneumocystis; Pneumocystis carinii - classification; Pneumocystis carinii - genetics; Pneumocystis jirovecii; Pneumonia, Pneumocystis - epidemiology; Pneumonia, Pneumocystis - microbiology; Polymerase Chain Reaction; RNA, Ribosomal - genetics; Sequence Analysis, DNA; Spain - epidemiology; genotypes; epidemiology; molecular analysis; cystic fibrosis; Carriage; Pneumocystis jirovecii; Human; Typing; Respiratory disease; Metabolic diseases; Genotype; Cystic fibrosis; Epidemiology; Genetic disease; Fungi; Infection; Polymerase chain reaction; Microbiological investigation; Digestive diseases; Pneumocystis; Fungi Imperfecti; Molecular biology; Colonization; Pancreatic disease; Thallophyta
• ISSN: 1198-743X; 1469-0691
• DOI: 10.1111/j.1469-0691.2007.01789.x

Although asymptomatic carriers of Pneumocystis jirovecii with cystic fibrosis (CF) have been described previously, the molecular epidemiology of P. jirovecii in CF patients has not yet been clarified. This study identified the distribution and dynamic evolution of P. jirovecii genotypes based on the mitochondrial large-subunit (mt LSU) rRNA gene. The mt LSU rRNA genotypes of P. jirovecii isolates in 33 respiratory samples from CF patients were investigated using nested PCR and direct sequencing. Three different genotypes were detected: 36.3% genotype 1 (85C/248C); 15.1% genotype 2 (85A/248C); 42.4% genotype 3 (85T/248C); and 6% mixed genotypes. Patients studied during a 1-year follow-up period showed a continuous colonisation/clearance cycle involving P. jirovecii and an accumulative tendency to be colonised with genotype 3.