Identification of a SulP-Type Bicarbonate Transporter in Marine Cyanobacteria

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 101; no. 52; pp. 18228 - 18233
• Main Authors: Price, G Dean, Woodger, Fiona J, Badger, Murray R, Howitt, Susan M, Tucker, Loraine
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28-12-2004; National Acad Sciences
• Subjects: Badgers; Bicarbonates - metabolism; Biological Sciences; Biological Transport; Carbon - chemistry; Carbon - pharmacology; Carbon dioxide; Carbon Dioxide - chemistry; Carbon Dioxide - metabolism; Cyanobacteria; Cyanobacteria - metabolism; Dose-Response Relationship, Drug; Evolution; Fresh water; Genes; Genetic Vectors; Genomes; Hydrogen-Ion Concentration; Kinetics; Mutation; Oxygen - metabolism; Photosynthesis; Phylogeny; Physiology; Plants; Reverse Transcriptase Polymerase Chain Reaction; Ribulose-Bisphosphate Carboxylase - pharmacology; Sea transportation; Silicon - chemistry; Sodium - chemistry; Sodium-Bicarbonate Symporters - chemistry; Sodium-Bicarbonate Symporters - physiology; Sulfates; Synechococcus - metabolism
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0405211101

Cyanobacteria possess a highly effective CO2-concentrating mechanism that elevates CO2 concentrations around the primary carboxylase, Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). This CO2-concentrating mechanism incorporates light-dependent, active uptake systems for CO2 and HCO3-. Through mutant studies in a coastal marine cyanobacterium, Synechococcus sp. strain PCC7002, we identified bicA as a gene that encodes a class of HCO3- transporter with relatively low transport affinity, but high flux rate. BicA is widely represented in genomes of oceanic cyanobacteria and belongs to a large family of eukaryotic and prokaryotic transporters presently annotated as sulfate transporters or permeases in many bacteria (SulP family). Further gain-of-function experiments in the freshwater cyanobacterium Synechococcus PCC7942 revealed that bicA expression alone is sufficient to confer a Na+-dependent, HCO3- uptake activity. We identified and characterized three cyanobacterial BicA transporters in this manner, including one from the ecologically important oceanic strain, Synechococcus WH8102. This study presents functional data concerning prokaryotic members of the SulP transporter family and represents a previously uncharacterized transport function for the family. The discovery of BicA has significant implications for understanding the important contribution of oceanic strains of cyanobacteria to global CO2 sequestration processes.