Immobilization of Pseudomonas fluorescens lipase on hydrophobic supports and application in biodiesel synthesis by transesterification of vegetable oils in solvent-free systems

• Published in: Journal of industrial microbiology & biotechnology Vol. 42; no. 4; pp. 523 - 535
• Main Authors: Lima, Lionete N, Oliveira, Gladson C, Rojas, Mayerlenis J, Castro, Heizir F, Da Rós, Patrícia C. M, Mendes, Adriano A, Giordano, Raquel L. C, Tardioli, Paulo W
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-04-2015; Springer Berlin Heidelberg; Oxford University Press
• Subjects: Adsorption; Agricultural biotechnology; babassu oil; Biocatalysis; Biocatalysts; Biochemistry; biodiesel; Biodiesel fuels; Biofuels; Biofuels - supply & distribution; Bioinformatics; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Catalysis; Chemical engineering; Energy consumption; Enzymes; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Esterification; Ethanol; ethanolysis; Genetic Engineering; Glycerol; Hydrogen-Ion Concentration; Hydrolysis; Hydrophobic and Hydrophilic Interactions; hydrophobicity; industrial microbiology; Inorganic Chemistry; Interfaces; Life Sciences; Lipase - chemistry; Lipase - metabolism; Microbiology; Plant Oils - chemistry; Plant Oils - metabolism; polystyrenes; Pore size; Pseudomonas fluorescens; Pseudomonas fluorescens - enzymology; Silica; Silicon Dioxide - chemistry; Solvents; Soybean oil; Soybean Oil - chemistry; Soybean Oil - metabolism; Soybeans; Studies; Temperature; transesterification; Triglycerides; vegetable oil; Vegetable oils; Vegetables; Brazil; Soy and babassu oils; Octyl-silica; Hydrophobic adsorption; Lipase; Biodiesel
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-015-1586-9

This work describes the preparation of biocatalysts for ethanolysis of soybean and babassu oils in solvent-free systems. Polystyrene, Amberlite (XAD-7HP), and octyl-silica were tested as supports for the immobilization of Pseudomonas fluorescens lipase (PFL). The use of octyl-silica resulted in a biocatalyst with high values of hydrolytic activity (650.0 ± 15.5 IU/g), immobilization yield (91.3 ± 0.3 %), and recovered activity (82.1 ± 1.5 %). PFL immobilized on octyl-silica was around 12-fold more stable than soluble PFL, at 45 °C and pH 8.0, in the presence of ethanol at 36 % (v/v). The biocatalyst provided high vegetable oil transesterification yields of around 97.5 % after 24 h of reaction using babassu oil and around 80 % after 48 h of reaction using soybean oil. The PFL-octyl-silica biocatalyst retained around 90 % of its initial activity after five cycles of transesterification of soybean oil. Octyl-silica is a promising support that can be used to immobilize PFL for subsequent application in biodiesel synthesis.