A Human Intestinal Cell Line Suitable for the Propagation of Human Parechovirus Type 1 to 6 with a Clear Cytopathic Effect

• Published in: Japanese Journal of Infectious Diseases Vol. 75; no. 3; pp. 318 - 321
• Main Authors: Takagi, Hirotaka, Oka, Tomoichiro, Ami, Yasushi, Suzaki, Yuriko, Saito, Hiroyuki
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 31-05-2022; Japan Science and Technology Agency
• Subjects: anti-HPeV-3 serum; Cell culture; Cell lines; cytopathic effect; human intestinal cell line; human parechovirus; Multiplicity of infection; Neutralization; neutralization assay; Nucleic acids; Pathogens; Propagation; Tissue culture; Viruses; human intestinal cell line; neutralization assay; cytopathic effect; human parechovirus; anti-HPeV-3 serum
• ISSN: 1344-6304; 1884-2836
• DOI: 10.7883/yoken.JJID.2021.534

Human parechoviruses (HPeVs) are being increasingly recognized as pathogens that cause mild-to-life-threatening diseases in children and adults. Recently, nucleic acid detection has become the mainstream method for pathogen detection. However, virus isolation is important for virus detection and further virologic characterization studies, and securing human pathogenic virus bioresources. We recently explored conventional cell lines suitable for human sapovirus isolation and accidentally identified a human duodenal cell line, HuTu80, that supported efficient growth of human parechovirus type 3 (HPeV-3) with clear cytopathic effects (CPE). Subsequently, we confirmed that all representative prototype HPeV type 1–6 strains were propagated efficiently in HuTu80 cells with clear CPE within 4 days. Another human ileocecal cell line, HCT-8 (HRT-18), also supports HPeV propagation except for HPeV-3. Titer values in HuTu80 and HCT-8 reached approximately 6.83–8.83 and 6.50–8.17 log10 50% tissue culture infectious dose/50 μL, respectively, when inoculated with multiplicity of infection of 0.0025. Previously reported cell lines likely support HPeV types 1–6 with different efficiency, especially for HPeV-3. In summary, HuTu80 can be used as an additional cell line for HPeV isolation, propagation with a clear CPE to produce a high titer value and for the virus neutralization assays.