CpG DNA as a Th1-promoting adjuvant in immunization against Trypanosoma cruzi
Th1-type immune response plays a critical role in resistance to Trypanosoma cruzi infection. We asked whether a synthetic oligodeoxynucleotide that contains immunostimulatory CpG motifs (CpG ODN), known to promote a Th1 response, could act as an adjuvant in immunization with parasite antigens. Mice...
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Published in: | Vaccine Vol. 19; no. 2; pp. 234 - 242 |
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Main Authors: | , |
Format: | Journal Article |
Language: | English |
Published: |
Oxford
Elsevier Ltd
15-09-2000
Elsevier |
Subjects: | |
Online Access: | Get full text |
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Summary: | Th1-type immune response plays a critical role in resistance to
Trypanosoma cruzi infection. We asked whether a synthetic oligodeoxynucleotide that contains immunostimulatory CpG motifs (CpG ODN), known to promote a Th1 response, could act as an adjuvant in immunization with parasite antigens. Mice immunized with a whole homogenate (WH) of
T. cruzi antigens co-administered with CpG ODN presented high titers of
T. cruzi antibodies (IgG2a isotype), strong delayed type hypersensitivity and a Th1-dominated (IFN-γ and IL-12) cytokine profile. Furthermore, WH plus CpG ODN protected mice from challenge with an otherwise lethal dose of bloodstream trypomastigotes. As reported for leishmaniasis and malaria, CpG ODN holds considerable promise as an adjuvant for future vaccines against
T. cruzi. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0264-410X 1873-2518 |
DOI: | 10.1016/S0264-410X(00)00172-9 |