CpG DNA as a Th1-promoting adjuvant in immunization against Trypanosoma cruzi

Th1-type immune response plays a critical role in resistance to Trypanosoma cruzi infection. We asked whether a synthetic oligodeoxynucleotide that contains immunostimulatory CpG motifs (CpG ODN), known to promote a Th1 response, could act as an adjuvant in immunization with parasite antigens. Mice...

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Published in:Vaccine Vol. 19; no. 2; pp. 234 - 242
Main Authors: Corral, Ricardo S., Petray, Patricia B.
Format: Journal Article
Language:English
Published: Oxford Elsevier Ltd 15-09-2000
Elsevier
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Summary:Th1-type immune response plays a critical role in resistance to Trypanosoma cruzi infection. We asked whether a synthetic oligodeoxynucleotide that contains immunostimulatory CpG motifs (CpG ODN), known to promote a Th1 response, could act as an adjuvant in immunization with parasite antigens. Mice immunized with a whole homogenate (WH) of T. cruzi antigens co-administered with CpG ODN presented high titers of T. cruzi antibodies (IgG2a isotype), strong delayed type hypersensitivity and a Th1-dominated (IFN-γ and IL-12) cytokine profile. Furthermore, WH plus CpG ODN protected mice from challenge with an otherwise lethal dose of bloodstream trypomastigotes. As reported for leishmaniasis and malaria, CpG ODN holds considerable promise as an adjuvant for future vaccines against T. cruzi.
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ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00172-9