Seed size plasticity in response to embryonic lethality conferred by ectopic CYCD activation is dependent on plant architecture

• Published in: Plant signaling & behavior Vol. 11; no. 7; p. e1192741
• Main Authors: Sornay, E., Dewitte, W., Murray, J. A. H.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 02-07-2016
• Subjects: Addendum; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; cell cycle; Cell Cycle - genetics; Cell Cycle - physiology; compensation; CYCDs; Endosperm - embryology; Endosperm - metabolism; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; nutrient allocation; Ovule - embryology; Ovule - metabolism; plant development; plasticity; seed number; seed size; Seeds - embryology; Seeds - metabolism; plant development; seed number; Arabidopsis; CYCDs; cell cycle; seed size; plasticity; compensation; nutrient allocation
• ISSN: 1559-2316; 1559-2324; 1559-2324
• DOI: 10.1080/15592324.2016.1192741

The size of seeds is the result of cell proliferation and growth in the three seed compartments: the embryo, endosperm and integuments. Targeting expression of the D-type cyclin CYCD7;1 to the central cell and early endosperm (FWA:CYCD7;1) triggered nuclear divisions and partial ovule abortion, reducing seed number in each silique and leading to increased seed size. A similar effect on seed size was observed with other segregating embryo lethal mutations, suggesting caution is needed in interpreting apparent seed size phenotypes. Here, we show that the positive effect of FWA:CYCD7;1 on Arabidopsis seed size is modulated by the architecture of the mother plant. Larger seeds were produced in FWA:CYCD7;1 lines with unmodified inflorescences, and also upon removal of side branches and axillary stems. This phenotype was absent from inflorescences with increased axillary floral stems produced by pruning of the main stem. Given this apparent confounding influence of resource allocation on transgenes effect, we conclude that plant architecture is a further important factor to consider in appraising seed phenotypes.