In vitro activities of antifungals alone and in combination with tigecycline against Candida albicans biofilms

• Published in: PeerJ (San Francisco, CA) Vol. 6; p. e5263
• Main Authors: Hacioglu, Mayram, Birteksoz Tan, Ayse Seher, Dosler, Sibel, Inan, Nese, Otuk, Gulten
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 25-07-2018; PeerJ, Inc; PeerJ Inc
• Subjects: Amphotericin B; Antifungal; Antifungal agents; Antimicrobial agents; Antiparasitic agents; Behavior; Biofilm; Biofilms; Candida albicans; Caspofungin; Chemotherapy; Drug dosages; Drug resistance; Echinocandins; Fluconazole; Genetic aspects; Infections; Infectious Diseases; Itraconazole; Laboratories; Medical equipment; Microbiology; Mycology; Physiological aspects; Planktonic cells; Tigecycline; XTT; Yeast; New York; Istanbul Turkey; Turkey; France; United States > US; Candida albicans; Biofilm; XTT; Antifungal; Tigecycline
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.5263

may form biofilms, which are thought to underlie the most recalcitrant infections.
In this study, activities of antifungal agents alone and in combination with tigecycline against planktonic cells and mature and developing biofilms of
isolates were evaluated.
Amphotericin B and echinocandins were found to be the most effective agents against mature biofilms, whereas the least effective agent was fluconazole. Furthermore, the most effective anti-fungal monotherapies against biofilm formation were amphotericin B and anidulafungin, and the least effective monotherapy was itraconazole. The combination of tigecycline and amphotericin B yielded synergistic effects, whereas combinations containing itraconazole yielded antagonist effects against planktonic cells. The combination of tigecycline and caspofungin exhibited maximum efficacy against mature biofilms, whereas combinations containing itraconazole exhibited minimal effects. Combinations of tigecycline with amphotericin B or anidulafungin were highly effective against
biofilm formation.
In summary, tigecycline was highly active against
particularly when combined with amphotericin B and echinocandins.