Role of long non-coding RNA-adducin 3 antisense RNA1 in liver fibrosis of biliary atresia

• Published in: Bioengineered Vol. 13; no. 3; pp. 6222 - 6230
• Main Authors: Ye, Yongqin, Wu, Weifang, Zheng, Jiachen, Zhang, Lihui, Wang, Bin
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01-03-2022
• Subjects: biliary atresia; Biliary Atresia - genetics; Calmodulin-Binding Proteins; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Humans; Infant, Newborn; Liver Cirrhosis - genetics; liver fibrosis; lnc-ADD3-AS1; Long non-coding RNA; LX-2 cell migration; LX-2 cell proliferation; Research Paper; RNA, Antisense - metabolism; RNA, Bacterial; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; biliary atresia; liver fibrosis; LX-2 cell proliferation; LX-2 cell migration; lnc-ADD3-AS1; Long non-coding RNA
• ISSN: 2165-5979; 2165-5987
• DOI: 10.1080/21655979.2022.2041321

Biliary atresia (BA) is a devastating liver disease in neonates. Liver fibrosis is regarded as a universal and prominent feature of BA. Studies have revealed that long non-coding RNAs (lncRNAs) regulate cellular processes during the development of liver fibrosis in BA. Long non-coding RNA-adducin 3 antisense RNA1 (lnc-ADD3-AS1) has been shown to increase susceptibility to BA. However, the role of lnc-ADD3-AS1 in liver fibrosis in BA remains unclear. Here, we investigated the role of lnc-ADD3-AS1 in the proliferation, migration, and apoptosis of the immortalized human hepatic stellate cell (HSC) line, LX-2. We successfully overexpressed and silenced lnc-ADD3-AS1 in LX-2 cells using adenovirus vectors and evaluated the proliferation of transfected cells using the Cell Counting Kit-8 (CCK8) assay. Cell apoptosis was detected using annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double staining and flow cytometry. We then analyzed cell migration by performing wound-scratch and transwell migration assays. Our results show that lnc-ADD3-AS1 significantly promoted LX-2 cell proliferation and attenuated apoptosis. More importantly, lncRNA-ADD3-AS1 significantly accelerated the migration of LX-2 cells. Our data indicated that lncRNA-ADD3-AS1 plays a role in the pathogenesis of liver fibrosis in patients with BA and may serve as a potential diagnostic marker for monitoring liver fibrosis in BA or as a therapeutic target for the disease.