The Effects of Baicalin on Myoglobinuric Acute Renal Failure in Rats

• Published in: Balkan medical journal Vol. 35; no. 1; pp. 68 - 76
• Main Authors: Yalçınkaya Yavuz, Özlem, Aydoğdu, Nurettin, Taştekin, Ebru, Süt, Necdet
• Format: Journal Article
• Language: English
• Published: Turkey Trakya Üniversitesi 20-01-2018; Aves Yayincilik Ltd. STI; Galenos Publishing; Galenos Publishing House
• Subjects: acute kidney injury; Acute Kidney Injury - blood; Acute Kidney Injury - chemically induced; Acute Kidney Injury - prevention & control; Animals; Baicalin; Catalase - metabolism; Creatine Kinase - blood; Flavonoids - pharmacology; free radicals; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Glycerol - administration & dosage; Glycerol - toxicity; Injections, Intramuscular; Injections, Intraperitoneal; Kidneys; Male; Malondialdehyde - metabolism; Myoglobinuria - chemically induced; Nitric oxide; nitric oxide synthase; Original; Random Allocation; Rats; Rats, Sprague-Dawley; Rodents; Studies; Tıp; Urea - blood; Türkiye; Edirne; United States > US; Turkey; free radicals; acute kidney injury; nitric oxide nitric oxide synthase; Baicalin; nitric oxide; nitric oxide synthase
• ISSN: 2146-3123; 2146-3131
• DOI: 10.4274/balkanmedj.2017.0040

Myoglobinuric acute kidney injury is a uremic syndrome that develops due to damage of skeletal muscle. Free radicals and nitric oxide play an important role in the pathogenesis of myoglobinuric acute kidney injury. Baicalin has multiple bioactivities, including antimicrobial, anti-inflammatory and antioxidant properties and is a potent free radical scavenger.
To investigate the nephroprotective mechanism of baicalin on myoglobinuric acute kidney injury.
Animal experimentation.
In our study, male Sprague Dawley rats were divided into 4 groups. Control (n=8), Baicalin (n=8), myoglobinuric acute kidney injury (n=10) and myoglobinuric acute kidney injury + baicalin (n=10). The rats were deprived of water for 24 hours before receiving intramuscular injection. The control and baicalin groups were injected intramuscularly with saline (8 ml/kg), and the myoglobinuric acute kidney injury and myoglobinuric acute kidney injury + baicalin groups were given 50% glycerol 8 ml/kg. One hour later, the control and myoglobinuric acute kidney injury groups received saline intraperitoneally, and the baicalin and myoglobinuric acute kidney injury + baicalin groups were given 200 mg/kg baicalin. Twenty-four hours after the glycerol injection, urine and blood samples were taken, and the kidneys of the rats were harvested under intraperitoneally injections of anaesthesia.
We found that the levels of creatinine, urea, nitric oxide, alanine transaminase, aspartate aminotransferase, creatine kinase in serum samples, malondialdehyde, nitric oxide, inducible nitric oxide synthase, and endothelial nitric oxide synthase concentrations in renal tissue were increased in the myoglobinuric acute kidney injury group compared with the control group (p<0.05). The nitric oxide and glutathione levels in the kidney were significantly decreased in the myoglobinuric acute kidney injury + baicalin group compared with the myoglobinuric acute kidney injury group (p<0.05). There were no significant differences between any other parameters.
Our results did not show any protective effect of baicalin on myoglobinuric acute kidney injury, possibly because the different effective factors in the pathogenesis of experimental myoglobinuric acute kidney injury used in this experiment deviate from other experimental models. Moreover, detailed studies are needed to clarify the effects of baicalin in different doses and treatment durations in glycerol-induced acute kidney injury model.