"Good Fences Make Good Neighbors": How does the Human Gut Microchip Unravel Mechanism of Intestinal Inflammation?

• Published in: Gut microbes Vol. 11; no. 3; pp. 581 - 586
• Main Authors: Shin, Woojung, Hackley, Landon A., Kim, Hyun Jung
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 03-05-2020; Taylor & Francis Group
• Subjects: Addendum; Animals; Barrier function; Disease model; Gastrointestinal Microbiome; Gut inflammation-on-a-chip; Gut-on-a-chip; Host Microbial Interactions; Host-microbiome interaction; Humans; Inflammation; Inflammation - microbiology; Intestinal Diseases - microbiology; Lab-On-A-Chip Devices; Mice; Microbiome; Microchip Analytical Procedures; Microphysiological system; Models, Biological; Oxidative stress; Probiotics; Probiotics - therapeutic use; Oxidative stress; Probiotics; Barrier function; Microphysiological system; Gut-on-a-chip; Inflammation; Host-microbiome interaction; Gut inflammation-on-a-chip; Disease model; Microbiome
• ISSN: 1949-0976; 1949-0984
• DOI: 10.1080/19490976.2019.1626684

A microengineered human gut-on-a-chip has demonstrated intestinal physiology, three-dimensional (3D) epithelial morphogenesis, and longitudinal host-microbiome interactions in vitro. The modular accessibility and modularity of the microphysiological gut-on-a-chip can lead to the identification of the seminal trigger in intestinal inflammation. By coupling microbial and immune cells in a spatiotemporal manner, we discovered that the maintenance of healthy epithelial barrier function is necessary and sufficient to demonstrate the homeostatic tolerance of the gut. Here, we highlight the breakthrough of our new disease model and discuss the future impact of investigating the etiology and therapeutic targets in the multifactorial inflammatory bowel disease.