OsTZF1, a CCCH-tandem zinc finger protein gene, driven under own promoter produces no pleiotropic effects and confers salt and drought tolerance in rice

• Published in: Plant signaling & behavior Vol. 17; no. 1; p. 2142725
• Main Authors: Ilyas, Muhammad, Hussain Shah, Safdar, Fujita, Yasunari, Maruyama, Kyonoshin, Nakashima, Kazuo, Yamaguchi-Shinozaki, Kazuko, Jan, Asad
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 31-12-2022; Taylor & Francis Group
• Subjects: CCCH-zinc finger protein; Droughts; Gene Expression Regulation, Plant - genetics; Oryza - metabolism; oxidative stress; Plants, Genetically Modified - metabolism; Research Paper; rice; RNA metabolism; senescence; Sodium Chloride - pharmacology; stress; Zinc Fingers - genetics; senescence; stress; CCCH-zinc finger protein; rice; oxidative stress; RNA metabolism
• ISSN: 1559-2316; 1559-2324; 1559-2324
• DOI: 10.1080/15592324.2022.2142725

Different abiotic stresses induce OsTZF1, a tandem CCCH-type zinc finger domain gene, in rice. Here, we report that transgenic rice plants overexpressing OsTZF1 under own promoter (P
OsTZF1
:OsTZF1-OX [for overexpression]) transferred to soil showed normal growth similar to vector control plants. The P
OsTZF1
:OsTZF1-OX produced normal leaves without any lesion mimic phenotype and exhibited normal seed setting. The P
OsTZF1
:OsTZF1-OX plants showed significantly increased tolerance to salt and drought stresses and enhanced post stress recovery. Microarray analysis revealed a total of 846 genes up-regulated and 360 genes down-regulated in P
OsTZF1
:OsTZF1-OX salt-treated plants. Microarray analysis of P
OsTZF1:
OsTZF1-OX plants showed the regulation of many abiotic stress tolerance genes. These results suggest that OsTZF1-OX under own promoter show abiotic stress tolerance and produces no pleiotropic effect on phenotype of transgenic rice plant.