Polymorphism detection of PRKG2 gene and its association with the number of thoracolumbar vertebrae and carcass traits in Dezhou donkey

• Published in: BMC genomic data Vol. 24; no. 1; p. 2
• Main Authors: Wang, Tianqi, Liu, Ziwen, Wang, Xinrui, Li, Yuhua, Akhtar, Faheem, Li, Mengmeng, Zhang, Zhenwei, Zhan, Yandong, Shi, Xiaoyuan, Ren, Wei, Huang, Bingjian, Wang, Changfa, Chai, Wenqiong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04-01-2023; BioMed Central; BMC
• Subjects: Analysis; Animals; Beef cattle; Carcass traits; Cyclic GMP-Dependent Protein Kinase Type II - genetics; Dezhou donkey; Donkeys; Equidae - genetics; Ethylenediaminetetraacetic acid; Genes; Genetic aspects; Genetic polymorphisms; Genetic research; Introns; Phenotype; Polymorphism, Single Nucleotide - genetics; PRKG2; Single nucleotide polymorphisms; SNPs; Spine; Thoracolumbar vertebrae; Vertebrae; China; SNPs; PRKG2; Thoracolumbar vertebrae; Carcass traits; Dezhou donkey
• ISSN: 2730-6844; 2730-6844
• DOI: 10.1186/s12863-022-01101-6

Previous studies have shown that the protein kinase cGMP-dependent 2 (PRKG2) gene is associated with dwarfism in humans, dogo Argentines, and Angus cattle, as well as with height and osteoblastogenesis in humans. Therefore, the PRKG2 gene was used as the target gene to explore whether this gene is associated with several thoracolumbar vertebrae and carcass traits in Dezhou donkeys.
In this study, fifteen SNPs were identified by targeted sequencing, all of which were located in introns of the PRKG2 gene. Association analysis illustrated that the g.162153251 G > A, g.162156524 C > T, g.162158453 C > T and, g.162163775 T > G were significantly different from carcass weight. g.162166224 G > A, g.162166654 T > A, g.162167165 C > A, g.162167314 A > C and, g.162172653 G > C were significantly associated with the number of thoracic vertebrae. g.162140112 A > G was significantly associated with the number and the length of lumbar vertebrae, and g.162163775 T > G was significantly associated with the total number of thoracolumbar vertebrae.
Overall, the results of this study suggest that PRKG2 gene polymorphism can be used as a molecular marker to breed high-quality Dezhou donkeys.