Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

• Published in: Microorganisms (Basel) Vol. 8; no. 5; p. 683
• Main Authors: Turbat, Adiyadolgor, Rakk, Dávid, Vigneshwari, Aruna, Kocsubé, Sándor, Thu, Huynh, Szepesi, Ágnes, Bakacsy, László, D Škrbić, Biljana, Jigjiddorj, Enkh-Amgalan, Vágvölgyi, Csaba, Szekeres, András
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07-05-2020; MDPI
• Subjects: Beans; Bioassays; Cancer; Carotenoids; Chlorophyll; Crop yield; Deoxyribonucleic acid; DNA; Endophytes; endophytic fungi; Fungi; Genera; Growth; Health aspects; Herbal medicine; High-performance liquid chromatography; Host plants; IAA (indole-acetic-acid) production; Identification methods; Indoleacetic acid; Legumes; Liquid chromatography; Medicinal plants; Metabolites; Mimosaceae; Photosynthesis; Physiological aspects; Plant growth; plant growth-promoting activities; Siderophores; Solubilization; Sophora flavescens; Tryptophan; Hungary; Mongolia; IAA (indole-acetic-acid) production; plant growth-promoting activities; endophytic fungi
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms8050683

Endophytic fungi in symbiotic association with their host plant are well known to improve plant growth and reduce the adverse effects of both biotic and abiotic stresses. Therefore, fungal endophytes are beginning to receive increased attention in an effort to find growth-promoting strains that could be applied to enhance crop yield and quality. In our study, the plant growth-promoting activities of endophytic fungi isolated from various parts of
(a medicinally important plant in Mongolia and China) have been revealed and investigated. Fungal isolates were identified using molecular taxonomical methods, while their plant growth-promoting abilities were evaluated in plate assays. Altogether, 15 strains were isolated, representing the genera
,
and
. Five of the isolates possessed phosphate solubilization activities and twelve secreted siderophores, while all of them were able to produce indoleacetic acid (IAA) in the presence or absence of tryptophan. The endogenous and exogenous accumulation of IAA were also monitored in liquid cultures using the HPLC-MS/MS technique to refine the plate assay results. Furthermore, for the highest IAA producer fungi, the effects of their extracts were also examined in plant bioassays. In these tests, the primary root lengths of the model
were increased in several cases, while the biomasses were significantly lower than the control IAA treatment. Significant alterations have also been detected in the photosynthetic pigment (chlorophyll-a, -b and carotenoids) content due to the fungal extract treatments, but these changes did not show any specific trends.