Caffeic acid phenethyl ester (CAPE) protects PC12 cells from MPP+ toxicity by inducing the expression of neuron-typical proteins

• Published in: Neurotoxicology (Park Forest South) Vol. 45; pp. 131 - 138
• Main Authors: Santos, Neife Aparecida Guinaim dos, Martins, Nádia Maria, Silva, Roberto de Barros, Ferreira, Rafaela Scalco, Sisti, Flávia Malvestio, Santos, Antonio Cardozo dos
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-12-2014; Elsevier
• Subjects: 1-Methyl-4-phenylpyridinium - toxicity; Animals; Biological and medical sciences; Caffeic Acids - therapeutic use; CAPE (caffeic acid phenethyl ester); Cell Death - drug effects; Cell Differentiation - drug effects; Dose-Response Relationship, Drug; GAP-43; GAP-43 Protein - metabolism; Medical sciences; Nerve Growth Factor - metabolism; Neurites - drug effects; Neuroprotection; Neuroprotective Agents - therapeutic use; PC12 Cells; Phenylethyl Alcohol - analogs & derivatives; Phenylethyl Alcohol - therapeutic use; Prevention and actions; Public health. Hygiene; Public health. Hygiene-occupational medicine; Rats; Synapsin I; Synapsins - metabolism; Synaptophysin; Synaptophysin - metabolism; Toxicology; Synapsin I; Synaptophysin; Neuroprotection; CAPE (caffeic acid phenethyl ester); GAP-43; Growth associated protein 43; Rat; Toxicity; Rodentia; In vitro; Prevention; Vertebrata; Cell line; Mammalia; Neuron
• ISSN: 0161-813X; 1872-9711
• DOI: 10.1016/j.neuro.2014.09.007

•Total inhibition of neuritogenesis is an early step in the neurotoxicity of MPP+.•CAPE induces neuritogenesis and synaptogenesis in non-NGF-stimulated PC12 cells.•CAPE increases the expression of GAP-43, synapsin and synaptophysin in PC12 cells.•CAPE inhibits the impairment of neuritogenesis and the cell death induced by MPP+.•This is the first study to associate the neuroprotection of CAPE with such effects.
Neurite loss is an early event in neurodegenerative diseases; therefore, the regeneration of the network of neurites constitutes an interesting strategy of treatment for such disorders. Neurotrophic factors play a critical role in neuronal regeneration, but their clinical use is limited by their inability to cross the blood brain barrier. Oxidative and inflammatory events are implicated in neurodegeneration and antioxidant compounds have been suggested as potential neuroprotectors. The protective potential of CAPE (caffeic acid phenethyl ester) has been shown in different models of neurotoxicity (in vitro and in vivo) and it has been associated with immune-modulatory, antioxidant and anti-inflammatory properties; however, other mechanisms might be involved. The present study demonstrates that CAPE protects PC12 cells from the cellular death induced by the dopaminergic neurotoxin MPP+ by increasing the network of neurites. Results showed that CAPE induced the formation, elongation and ramification of neurites in PC12 cells non-stimulated with NGF (nerve growth factor) and inhibited the shortage of neurites induced by the dopaminergic neurotoxin. These effects were associated with increased expression of neuron-typical proteins responsible for axonal growth (GAP-43) and synaptogenesis (synaptophysin and synapsin I). It is noteworthy that, unlike neurotrophins, CAPE would be able to cross the blood brain barrier and exert its neurotrophic effects in the brain. This study corroborates the therapeutic potential of CAPE in neurodegenerative diseases while proposes the involvement of neuroplasticity in the mechanism of neuroprotection.