Heat stress causes alterations in the cell-wall polymers and anatomy of coffee leaves (Coffea arabica L.)

• Published in: Carbohydrate polymers Vol. 93; no. 1; pp. 135 - 143
• Main Authors: Lima, Rogério Barbosa, dos Santos, Tiago Benedito, Vieira, Luiz Gonzaga Esteves, Ferrarese, Maria de Lourdes Lúcio, Ferrarese-Filho, Osvaldo, Donatti, Lucélia, Boeger, Maria Regina Torres, Petkowicz, Carmen Lúcia de Oliveira
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier Ltd 01-03-2013; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Applied sciences; arabinose; Arabinose - chemistry; Biological and medical sciences; Cell wall; Cell Wall - chemistry; cell walls; chloroplasts; Chromatography, Gel - methods; Coffea - anatomy & histology; Coffea - chemistry; Coffea arabica; Coffee leaves; Economic plant physiology; EDTA (chelating agent); Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Galactans - chemistry; galactose; glucose; Heat stress; Hot Temperature; leaves; Lignin - chemistry; microscopy; Natural polymers; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Pectins - chemistry; Physicochemistry of polymers; Plant Cells - chemistry; Plant Leaves - anatomy & histology; polymers; Polysaccharides; Polysaccharides - chemistry; sodium hydroxide; Starch and polysaccharides; Stress, Physiological; uronic acids; Uronic Acids - chemistry; Water - chemistry; xylose; Polysaccharides; Coffee leaves; Heat stress; Coffea arabica; Cell wall; Thermal stress; Hemicellulose; Cellulose; Plant leaf; Pectin; Experimental study; Dicotyledones; Angiospermae; Rubiaceae; Internal structure; Oside polymer; Spermatophyta; Chemical composition; Cultivated plant
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.05.015

► Yields, monosaccharide composition and molar mass profiles of cell-wall polysaccharides from coffee leaves changes when submitted to heat stress. ► The primary monolignol contents in coffee leaves increased after the heat stress. ► Cell anatomy of coffee leaves change under heat stress.
Coffee plants were subjected to heat stress (37°C) and compared with control plants (24°C). Cell wall polysaccharides were extracted using water (W), EDTA (E) and 4M NaOH (H30 and H70). In addition, monolignols were analyzed, and the leaves were observed by microscopy. Plants under heat stress accumulated higher contents of arabinose and galactose in fraction W. Xylose contents were observed to decrease in H30 fractions after the heat stress, whereas galactose and uronic acid increased. H70 fractions from plants exposed to heat stress showed increased xylose contents, whereas the contents of arabinose and glucose decreased. Differences in the molar-mass profiles of polysaccharides were also observed. The primary monolignol contents increased after the heat stress. Structural alterations in palisade cells and ultrastructural damage in chloroplasts were also observed. Our results demonstrate that the chemical profile of coffee cell-wall polymers and structural cell anatomy change under heat stress.