Early pharmacotherapy restores neurogenesis and cognitive performance in the Ts65Dn mouse model for Down syndrome

• Published in: The Journal of neuroscience Vol. 30; no. 26; pp. 8769 - 8779
• Main Authors: Bianchi, Patrizia, Ciani, Elisabetta, Guidi, Sandra, Trazzi, Stefania, Felice, Daniela, Grossi, Gabriele, Fernandez, Mercedes, Giuliani, Alessandro, Calzà, Laura, Bartesaghi, Renata
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 30-06-2010
• Subjects: Animals; Animals, Newborn; Brain - drug effects; Brain - physiopathology; Brain-Derived Neurotrophic Factor - metabolism; Cell Proliferation - drug effects; Cognition - drug effects; Disease Models, Animal; Down Syndrome - drug therapy; Down Syndrome - physiopathology; Fluoxetine - administration & dosage; Fluoxetine - pharmacology; Memory Disorders - drug therapy; Memory Disorders - physiopathology; Mice; Mice, Transgenic; Neurogenesis - drug effects; Neurons - drug effects; Neurons - physiology; Receptor, Serotonin, 5-HT1A - metabolism; Serotonin - metabolism; Serotonin Uptake Inhibitors - administration & dosage; Serotonin Uptake Inhibitors - pharmacology; Stem Cells - drug effects; Stem Cells - physiology; Time Factors
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.0534-10.2010

Down syndrome (DS) is a genetic pathology characterized by intellectual disability and brain hypotrophy. Widespread neurogenesis impairment characterizes the fetal and neonatal DS brain, strongly suggesting that this defect may be a major determinant of mental retardation. Our goal was to establish, in a mouse model for DS, whether early pharmacotherapy improves neurogenesis and cognitive behavior. Neonate Ts65Dn mice were treated from postnatal day (P) 3 to P15 with fluoxetine, an antidepressant that inhibits serotonin (5-HT) reuptake and increases proliferation in the adult Ts65Dn mouse (Clark et al., 2006). On P15, they received a BrdU injection and were killed after either 2 h or 1 month. Results showed that P15 Ts65Dn mice had notably defective proliferation in the hippocampal dentate gyrus, subventricular zone, striatum, and neocortex and that proliferation was completely rescued by fluoxetine. In the hippocampus of untreated P15 Ts65Dn mice, we found normal 5-HT levels but a lower expression of 5-HT1A receptors and brain-derived neurotrophic factor (BDNF). In Ts65Dn mice, fluoxetine treatment restored the expression of 5-HT1A receptors and BDNF. One month after cessation of treatment, there were more surviving cells in the dentate gyrus of Ts65Dn mice, more cells with a neuronal phenotype, more proliferating precursors, and more granule cells. These animals were tested for contextual fear conditioning, a hippocampus-dependent memory task, and exhibited a complete recovery of memory performance. Results show that early pharmacotherapy with a drug usable by humans can correct neurogenesis and behavioral impairment in a model for DS.