Association between SNPs of Circulating Vascular Endothelial Growth Factor Levels, Hypercholesterolemia and Metabolic Syndrome

• Published in: Medicina (Kaunas, Lithuania) Vol. 55; no. 8; p. 464
• Main Authors: Salami, Ali, El Shamieh, Said
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 11-08-2019; MDPI AG
• Subjects: Adult; Cross-Sectional Studies; Endothelial Growth Factors - genetics; Female; Humans; hypercholesterolemia; Hypercholesterolemia - genetics; Iran; Male; metabolic syndrome; Metabolic Syndrome - genetics; Middle Aged; Polymorphism, Single Nucleotide - genetics; Receptors, LDL - analysis; Receptors, LDL - blood; single nucleotide polymorphisms; VEGF; Iran; metabolic syndrome; single nucleotide polymorphisms; VEGF; hypercholesterolemia
• ISSN: 1648-9144; 1010-660X; 1648-9144; 1010-660X
• DOI: 10.3390/medicina55080464

Four single nucleotide polymorphisms (SNPs); rs6921438 and rs4416670 in
-
, rs6993770 in
and rs10738760 in
-
were reported to explain up to 50% of the heritability of vascular endothelial growth factor circulating levels. These SNPs were also studied for possible associations with circulating lipid levels in supposedly healthy European individuals and in a limited number of Iranian individuals with metabolic syndrome. To go further, the association of those four SNPs with plasma lipid parameters, hypercholesterolemia and metabolic syndrome (MetS) was assessed.
A cross-sectional study was conducted on 460 individuals chosen from the general population. Demographic and clinical data were collected and DNA was extracted and genotyped using Kompetitive allele specific PCR (KASP™). A meta-analysis followed, combining our participants with the Iranian individuals (
= 336).
Whereas rs10738760 was associated with total cholesterol (Tchol) (
= 0.01), rs6993770 showed significant associations with both Tchol and low-density lipoprotein cholesterol (LDL-C) levels (
= 0.007 and
= 0.01 respectively). Using a multivariate logistic regression model adjusted for different confounding factors, we found that rs6993770 was associated with hypercholesterolemia, specifically high Tchol (
= 0.01) and LDL-C levels (
= 0.01). Furthermore, rs10738760 was positively associated with the risk of MetS in these individuals (
= 0.02) and in the meta-analysis (OR = 1.67,
= 0.01).
Our results suggest that whereas rs6993770 in
was positively associated with hypercholesterolemia, rs10738760 (
-
) has a possible implication in MetS in two Middle Eastern populations.