Low-dose, simple, and fast grating-based X-ray phase-contrast imaging

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 107; no. 31; pp. 13576 - 13581
• Main Authors: Zhu, Peiping, Zhang, Kai, Wang, Zhili, Liu, Yijin, Liu, Xiaosong, Wu, Ziyu, McDonald, Samuel A., Marone, Federica, Stampanoni, Marco, Bai, Chunli
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 03-08-2010; National Acad Sciences
• Subjects: Animals; Field of view; Imaging; Interferometers; Light refraction; Mice; Optics; Phase contrast imaging; Physical Sciences; Radiation Dosage; Radiography; Radiography - methods; Rats; Refraction; Time Factors; Tomography; Tomography, X-Ray - methods; X ray sources; X-rays
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1003198107

Phase sensitive X-ray imaging methods can provide substantially increased contrast over conventional absorption-based imaging and therefore new and otherwise inaccessible information. The use of gratings as optical elements in hard X-ray phase imaging overcomes some of the problems that have impaired the wider use of phase contrast in X-ray radiography and tomography. So far, to separate the phase information from other contributions detected with a grating interferometer, a phase-stepping approach has been considered, which implies the acquisition of multiple radiographic projections. Here we present an innovative, highly sensitive X-ray tomographic phase-contrast imaging approach based on grating interferometry, which extracts the phase-contrast signal without the need of phase stepping. Compared to the existing phase-stepping approach, the main advantages of this new method dubbed "reverse projection" are not only the significantly reduced delivered dose, without the degradation of the image quality, but also the much higher efficiency. The new technique sets the prerequisites for future fast and low-dose phase-contrast imaging methods, fundamental for imaging biological specimens and in vivo studies.