Tomographic molecular imaging and 3D quantification within adult mouse organs

• Published in: Nature methods Vol. 4; no. 1; pp. 31 - 33
• Main Authors: Sharpe, James, Ahlgren, Ulf, Alanentalo, Tomas, Asayesh, Amir, Morrison, Harris, Lorén, Christina E, Holmberg, Dan
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01-01-2007
• Subjects: Animals; Biomedical research; Diabetes; Diabetes Mellitus, Type 1 - diagnosis; Diabetes Mellitus, Type 1 - physiopathology; Diagnosis; Diagnostic imaging; Disease Models, Animal; Disease Progression; Image Processing, Computer-Assisted - methods; Imaging, Three-Dimensional - methods; Islets of Langerhans - pathology; Methods; Mice; Molecular structure; Pancreas - pathology; Rodents; Sample preparation; Sensitivity and Specificity; Three dimensional imaging; Tomography; Tomography - methods; Type 1 diabetes; Spain; Disease Models; Type 1/diagnosis/physiopathology; Animal; Image Processing; Imaging; Diabetes Mellitus; Computer-Assisted/methods; Three-Dimensional/methods
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/nmeth985

A convenient technology to quantify three-dimensional (3D) morphological features would have widespread applications in biomedical research. Based on combined improvements in sample preparation, tomographic imaging and computational processing, we present a procedure for high-resolution 3D quantification of structures within intact adult mouse organs. Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet β-cell volume and the onset of type-1 diabetes.