First isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil

• Published in: BMC microbiology Vol. 20; no. 1; p. 360
• Main Authors: Alves, Vinicius Buiatte de Andrade, Carvalho, Eneas, Madureira, Paloma Alonso, Marino, Elizangela Domenis, Vaz, Andreia Cristina Nakashima, Vidal, Ana Maria Centola, Ruiz, Vera Letticie de Azevedo
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 24-11-2020; BioMed Central
• Subjects: Algae; Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Bacteria; Deoxyribonucleic acid; DNA; DNA sequencing; Epidemiology; Genetic aspects; Genomes; Gram-negative bacterial infections; Health aspects; Health risks; High resistance; Identification; Identification and classification; Infections; Livestock; Livestock farms; Nucleotide sequencing; Public health; Risk factors; Salmonella; Shewanella; Swine; Taxonomy; Whole genome sequencing; Brazil; France; Resistance; Genome; Shewanella; Swine; Taxonomy
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-020-02040-x

Infections caused by Shewanella spp. have been increasingly reported worldwide. The advances in genomic sciences have enabled better understanding about the taxonomy and epidemiology of this agent. However, the scarcity of DNA sequencing data is still an obstacle for understanding the genus and its association with infections in humans and animals.
In this study, we report the first isolation and whole-genome sequencing of a Shewanella algae strain from a swine farm in Brazil using the boot sock method, as well as the resistance profile of this strain to antimicrobials. The isolate was first identified as Shewanella putrefaciens, but after whole-genome sequencing it showed greater similarity with Shewanella algae. The strain showed resistance to 46.7% of the antimicrobials tested, and 26 resistance genes were identified in the genome.
This report supports research made with Shewanella spp. and gives a step forward for understanding its taxonomy and epidemiology. It also highlights the risk of emerging pathogens with high resistance to antimicrobial formulas that are important to public health.