The structure of BVU2987 from Bacteroides vulgatus reveals a superfamily of bacterial periplasmic proteins with possible inhibitory function

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 66; no. 10; pp. 1265 - 1273
• Main Authors: Das, Debanu, Finn, Robert D., Carlton, Dennis, Miller, Mitchell D., Abdubek, Polat, Astakhova, Tamara, Axelrod, Herbert L., Bakolitsa, Constantina, Chen, Connie, Chiu, Hsiu-Ju, Chiu, Michelle, Clayton, Thomas, Deller, Marc C., Duan, Lian, Ellrott, Kyle, Ernst, Dustin, Farr, Carol L., Feuerhelm, Julie, Grant, Joanna C., Grzechnik, Anna, Han, Gye Won, Jaroszewski, Lukasz, Jin, Kevin K., Klock, Heath E., Knuth, Mark W., Kozbial, Piotr, Krishna, S. Sri, Kumar, Abhinav, Marciano, David, McMullan, Daniel, Morse, Andrew T., Nigoghossian, Edward, Nopakun, Amanda, Okach, Linda, Puckett, Christina, Reyes, Ron, Rife, Christopher L., Sefcovic, Natasha, Tien, Henry J., Trame, Christine B., Van Den Bedem, Henry, Weekes, Dana, Wooten, Tiffany, Xu, Qingping, Hodgson, Keith O., Wooley, John, Elsliger, Marc-André, Deacon, Ashley M., Godzik, Adam, Lesley, Scott A., Wilson, Ian A.
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01-10-2010; Wiley Subscription Services, Inc
• Subjects: Amino Acid Sequence; Bacteria; Bacteroides - chemistry; Bacteroides - metabolism; Bacteroides vulgatus; BASIC BIOLOGICAL SCIENCES; beta -Lactamase; biochemistry & molecular biology; biophysics; BVU2987; Conserved Sequence; Crystal structure; crystallography; Crystallography, X-Ray; Digestive tract; DUF2874; Human Gut Microbiome; Models, Molecular; Molecular Sequence Data; Oral cavity; Periplasmic Proteins - chemistry; Periplasmic Proteins - metabolism; PF11396; Protein Binding; Protein Structure, Tertiary; putative inhibitor proteins; Sequence Alignment; Structural Homology, Protein; β-lactamase inhibitor protein-like fold
• ISSN: 1744-3091; 1744-3091; 2053-230X
• DOI: 10.1107/S1744309109046788

Proteins that contain the DUF2874 domain constitute a new Pfam family PF11396. Members of this family have predominantly been identified in microbes found in the human gut and oral cavity. The crystal structure of one member of this family, BVU2987 from Bacteroides vulgatus, has been determined, revealing a β‐lactamase inhibitor protein‐like structure with a tandem repeat of domains. Sequence analysis and structural comparisons reveal that BVU2987 and other DUF2874 proteins are related to β‐lactamase inhibitor protein, PepSY and SmpA_OmlA proteins and hence are likely to function as inhibitory proteins.