Comparative genome analysis and molecular epidemiology of the reemerging porcine epidemic diarrhea virus strains isolated in Korea

• Published in: Infection, genetics and evolution Vol. 26; pp. 348 - 351
• Main Authors: Choi, Jong-Chul, Lee, Kun-Kyu, Pi, Jae Ho, Park, Seung-Yong, Song, Chang-Seon, Choi, In-Soo, Lee, Joong-Bok, Lee, Dong-Hun, Lee, Sang-Won
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-08-2014
• Subjects: Animal virus; Animals; Communicable Diseases, Emerging; Complete genome; Coronavirus; Coronavirus Infections - veterinary; Genome, Viral; Genomics; Phylogenetic analysis; Phylogeny; Porcine epidemic diarrhea virus; Porcine epidemic diarrhea virus - genetics; Porcine epidemic diarrhea virus - isolation & purification; Republic of Korea - epidemiology; RNA, Viral - genetics; Sequence Analysis, DNA; Swine; Swine Diseases - epidemiology; Swine Diseases - virology; Republic of Korea; Phylogenetic analysis; Animal virus; Porcine epidemic diarrhea virus; Complete genome; Coronavirus
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2014.06.005

•Porcine epidemic diarrhea virus has been reemerged at November 2013 in Korea.•The reemerging Korean PEDV has been completely sequenced.•Complete genome sequences were highly diverge between new and old Korean PEDVs.•The reemerging Korean PEDV is practically identical to the USA PEDV.
Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family, is an enveloped, positive-sense, single-stranded RNA virus, which causes severe diarrhea and dehydration in suckling pigs. We detected three PEDV strains from ten small intestine samples from piglets with acute diarrhea and we determined the complete genome sequences of the reemerging Korean PEDV field isolates, except for the noncoding regions from both ends. The complete genome sequences of the strains were identical or almost identical (one synonymous single-nucleotide polymorphism (SNP) in the ORF1a/1b genomic sequence). Interestingly, comparative genome analysis of recent Korean PEDV isolates and other strains revealed that the complete genome sequences of recent Korean strains were almost identical (99.9%) to those of the US PEDV strains isolated in 2013. These results suggest that the three reemerging Korean strains are distinct from previous endemic Korean PEDV strains and has been recently introduced into Korea from oversea with high likelihood.