Influence of the route of administration on immunomodulatory properties of bovine β-lactoglobulin-producing Lactobacillus casei

• Published in: Vaccine Vol. 27; no. 42; pp. 5800 - 5805
• Main Authors: Hazebrouck, S, Przybylski-Nicaise, L, Ah-Leung, S, Adel-Patient, K, Corthier, G, Langella, P, Wal, J.-M
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 25-09-2009; Elsevier
• Subjects: Administration, Intranasal; Administration, Oral; Allergy and Immunology; Animals; Applied microbiology; Bacteriology; Biological and medical sciences; Cattle; Cytokines - immunology; Desensitization, Immunologic - methods; Feces; Female; Fundamental and applied biological sciences. Psychology; Immunoglobulin A - immunology; Immunoglobulin E - analysis; Immunoglobulin E - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Immunologic Factors - immunology; Intranasal and oral applications; Lactobacillus casei; Lactobacillus casei - immunology; Lactoglobulins - immunology; Life Sciences; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Recombinant allergen; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); β-lactoglobulin; Recombinant allergen; Intranasal and oral applications; Mice; Lactobacillus casei; β-lactoglobulin; Immunopathology; Allergy; Lactic acid bacteria; Bovine; Rodentia; Intranasal administration; Route of administration; Vertebrata; Mammalia; Mouse; Bacteria; Lactobacillaceae; Artiodactyla; Veterinary; Ungulata; Allergen; Lactobacillus; beta lactoglobulin; intranasal and oral application; recombinant allergen; mice
• ISSN: 0264-410X; 1873-2518; 0264-410X
• DOI: 10.1016/j.vaccine.2009.07.064

Abstract Because of their intrinsic immunomodulatory properties, some lactic acid bacteria were reported to modulate allergic immune responses in mice and humans. We recently developed recombinant strains of Lactobacillus casei that produce β-lactoglobulin (BLG), a major cow's milk allergen. Here, we investigated immunomodulatory potency of intranasal and oral administrations of recombinant lactobacilli on a subsequent sensitization of mice to BLG. Intranasal administration of the BLG-producing Lb. casei stimulated serum BLG-specific IgG2a and IgG1 responses, and fecal IgA response as well, but did not inhibit BLG-specific IgE production. In contrast, oral administration led to a significant inhibition of BLG-specific IgE production while IgG1 and IgG2a responses were not stimulated. After both oral and intranasal administrations, production of IL-17 cytokine by BLG-reactivated splenocytes was similarly enhanced, thus confirming the adjuvant effect of the Lb. casei strain. However, a mixed Th1/Th2 cell response was evidenced in BLG-reactivated splenocytes from mice intranasally pretreated, with enhanced secretions of Th1 cytokines (IFN-γ and IL-12) and Th2 cytokines (IL-4 and IL-5) whereas only production of Th1 cytokines, but not Th2 cytokines, was enhanced in BLG-reactivated splenocytes from mice orally pretreated. Our results show that the mode of administration of live bacteria may be critical for their immunomodulatory effects.