Oxidation of unsaturated phospholipids in membrane bilayer mixtures is accompanied by membrane fluidity changes

• Published in: Biochimica et biophysica acta Vol. 1487; no. 1; pp. 61 - 73
• Main Authors: Borst, Jan Willem, Visser, Nina V, Kouptsova, Olga, Visser, Antonie J.W.G
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24-08-2000
• Subjects: Arachidonic acid; Biochemie; Biochemistry; BODIPY-, DPH- and pyrene-probe; Diffusion; Fluorescence correlation spectroscopy; Fluorescence Polarization; Fluorescent Dyes - chemistry; Lateral diffusion; Lipid Bilayers - chemistry; Lipid oxidation; Liposome; Liposomes - chemistry; Membrane Fluidity; Membrane Lipids - chemistry; Molecular Structure; Oxidation-Reduction; Phospholipids - chemistry; Spectrometry, Fluorescence; Unsaturated phospholipid; VLAG; SAPC, 1-stearoyl-2-arachidonoyl- sn-glycero-3-phosphocholine; DMPC, 1,2-dimyristoyl- sn-glycero-3-phosphocholine; Lateral diffusion; diPyr 10-PC, 1,2-bis-(1-pyrenedecanoyl)- sn-glycero-3-phosphocholine; Liposome; SUV’s, small unilamellar vesicles; Arachidonic acid; BP-C11, 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza- s-indacene-3-undecanoic acid; BP-C5-HPC, 2-(4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza- s-indacene-3-pentanoyl)-1-hexadecanoyl- sn-glycero-3-phosphocholine; DOPC, 1,2-dioleoyl- sn-glycero-3-phosphocholine; Membrane fluidity; DPH-HPC, 2-(3-(diphenylhexatrienyl)propanoyl)-1-hexadecanoyl- sn-glycero-3-phosphocholine; E/M, ratio of excimer and monomer fluorescence intensities; BODIPY-, DPH- and pyrene-probe; monoPyr 10-HPC, 1-hexadecanoyl-2-(1-pyrenedecanoyl)- sn-glycero-3-phosphocholine; Lipid oxidation; Unsaturated phospholipid; FCS, fluorescence correlation spectroscopy; diPyr 4-PC, 1,2-bis-(1-pyrenebutanoyl)- sn-glycero-3-phosphocholine; Fluorescence correlation spectroscopy
• ISSN: 1388-1981; 0006-3002; 1879-2618
• DOI: 10.1016/S1388-1981(00)00084-6

Steady-state and time-resolved fluorescence spectroscopy has been used to obtain information on oxidation processes and associated dynamical and structural changes in model membrane bilayers made from single unilamellar vesicles (SUV’s) of 1,2-dioleoyl-
sn-glycero-3-phosphocholine (DOPC) mixed with increasing amounts of 1-stearoyl-2-arachidonoyl-
sn-glycero-3-phosphocholine (SAPC). The highly unsaturated arachidonoyl chain containing four double bonds is prone to oxidation. Lipid oxidation was initiated chemically by a proper oxidant and could be followed on line via the fluorescence changes of an incorporated fluorescent lipophilic fatty acid: 4,4-difluoro-5-(4-phenyl-1,3-butadienyl)-4-bora-3a,4a-diaza-
s-indacene-3-undecanoic acid (BP-C11). The oxidation rate increases with an increasing amount of SAPC. Size measurements of different SUV’s incorporated with a trace amount of a phosphatidylcholine analogue of BP-C11 using fluorescence correlation spectroscopy have demonstrated that an increase of lipid unsaturation results in smaller sized SUV’s and therefore to a larger curvature of the outer bilayer leaflet. This suggests that the lipid–lipid spacing has increased and that the unsaturated fatty acyl chains are better accessible for the oxidant. Oxidation results in some characteristic physical changes in membrane dynamics and structure, as indicated by the use of specific fluorescence probes. Fluorescence measurements of both dipyrenyl- and diphenylhexatriene-labelled PC introduced in non-oxidised and oxidised DOPC–SAPC membranes clearly show that the microfluidity (local fluidity at the very site of the probes) significantly decreases when the oxidised SAPC content increases in the lipid mixture. A similar effect is observed from the lateral diffusion experiments using monopyrenyl PC in the same membrane systems: the lateral diffusion is distinctly slower in oxidised membranes.