Donor‐Plant Environment Effects on Regeneration from Barley Embryo‐Derived Callus

• Published in: Crop science Vol. 39; no. 3; pp. 682 - 685
• Main Author: Dahleen, Lynn S.
• Format: Journal Article
• Language: English
• Published: Madison, WI Crop Science Society of America 01-05-1999; American Society of Agronomy
• Subjects: Adaptation to environment and cultivation conditions; Agricultural research; Agronomy. Soil science and plant productions; Analysis; Barley; Biological and medical sciences; Biotechnology; Embryos; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetics; Genetics and breeding of economic plants; Grain; Hordeum vulgare; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Plant cells and fungal cells; Plant genetics; Plant growth; Plant reproduction; Varietal selection. Specialized plant breeding, plant breeding aims; Culture medium; Regeneration; Genetic transformation; Genotype environment interaction; Tissue culture; Micropropagation; Intraspecific comparison; Optimization
• ISSN: 0011-183X; 1435-0653
• DOI: 10.2135/cropsci1999.0011183X003900020013x

High levels of green plant regeneration are necessary for efficient barley (Hordeum vulgare L.) transformation. Experiments to optimize regeneration of calli derived from immature embryos have examined the effects of genotype and media components, but research on the effects of donor‐plant growth environment is lacking. Defined growth conditions in growth chambers are known to give consistent regeneration rates, but space often is limited. The objective of this project was to evaluate regeneration from barley tissue cultures of ‘Golden Promise’ and ‘Morex’ grown under greenhouse conditions, planted on four different dates, to determine optimal greenhouse planting times. Results were compared with cultures from plants grown in the growth chamber planted on the same four dates. Genotype, growth environment, and planting date had significant effects on green plant regeneration. Cultures of embryos from plants sown in the greenhouse in December resulted in significantly fewer green regenerants per plate. Regeneration was more dependent on the average solar radiation than on temperature. The best greenhouse regeneration rates for Morex were not significantly different than regeneration from growth chamber‐grown donor plants. Green plant regeneration was higher and less variable from growth chamber‐grown donor plants. Sufficient regeneration from greenhouse‐grown donor plants can be reached when natural light levels are high and temperatures are moderate.