Antimicrobial activity and safety evaluation of peptides isolated from the hemoglobin of chickens

• Published in: BMC microbiology Vol. 16; no. 1; p. 287
• Main Authors: Hu, Fengjiao, Wu, Qiaoxing, Song, Shuang, She, Ruiping, Zhao, Yue, Yang, Yifei, Zhang, Meikun, Du, Fang, Soomro, Majid Hussain, Shi, Ruihan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 05-12-2016; BioMed Central
• Subjects: Analysis; Animals; Anti-Bacterial Agents - adverse effects; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Anti-infective agents; Anti-Infective Agents - isolation & purification; Anti-Infective Agents - pharmacology; Antibiotics; Antimicrobial agents; Antimicrobial Cationic Peptides - isolation & purification; Antimicrobial Cationic Peptides - pharmacology; Bacteria; Bacteria - drug effects; Bacteria - growth & development; Bacteriolysis - drug effects; Biofilms - drug effects; Biofilms - growth & development; Blood Bactericidal Activity; Cell Membrane Permeability - drug effects; Chickens; Drug resistance in microorganisms; Drug Resistance, Multiple, Bacterial; E coli; Gram-positive bacteria; Health aspects; Hemoglobin; Hemoglobins - chemistry; Hemoglobins - pharmacology; Hemolytic Agents - isolation & purification; Hemolytic Agents - pharmacology; Laboratories; Microbial Sensitivity Tests; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Peptide Fragments - chemistry; Peptide Fragments - pharmacology; Peptides; Poultry; Protein Stability; Sodium; Sodium Chloride - metabolism; Veterinary colleges; Veterinary medicine; Beijing China; United States > US; Massachusetts; China; Hydrolysis; Hemoglobin; Antimicrobial activity; Bactericidal activity; Antimicrobial peptides
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-016-0904-3

Hemoglobin is a rich source of biological peptides. As a byproduct and even wastewater of poultry-slaughtering facilities, chicken blood is one of the most abundant source of hemoglobin.
In this study, the chicken hemoglobin antimicrobial peptides (CHAP) were isolated and the antimicrobial and bactericidal activities were tested by the agarose diffusion assay, minimum inhibitory concentration (MIC) analysis, minimal bactericidal concentration (MBC) analysis, and time-dependent inhibitory and bactericidal assays. The results demonstrated that CHAP had potent and rapid antimicrobial activity against 19 bacterial strains, including 9 multidrug-resistant bacterial strains. Bacterial biofilm and NaCl permeability assays, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were further performed to detect the mechanism of its antimicrobial effect. Additionally, CHAP showed low hemolytic activity, embryo toxicity, and high stability in different temperatures and animal plasma.
CHAP may have great potential for expanding production and development value in animal medication, the breeding industry and environment protection.