Stromal low temperature compartment derived from the inner membrane of the chloroplast envelope

• Published in: Plant physiology (Bethesda) Vol. 97; no. 4; pp. 1558 - 1564
• Main Authors: Morre, D.J. (Purdue University, West Lafayette, IN), Sellden, G, Sundqvist, C, Sandelius, A.S
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-12-1991
• Subjects: ANATOMIA DE LA PLANTA; ANATOMIE VEGETALE; Biological and medical sciences; Cell biochemistry; Cell membranes; Cell physiology; characterization; CHLOROPLASTE; Chloroplasts; CLOROPLASTO; compartments; Endoplasmic reticulum; ESTRUCTURA CELULAR; FEUILLE; Fundamental and applied biological sciences. Psychology; GLYCINE MAX; HOJAS; Lipids; Low temperature; NICOTIANA TABACUM; Peas; PISUM SATIVUM; Plant physiology and development; Plants; PLASTE; PLASTIDIOS; Plastids; Spinach; SPINACIA OLERACEA; STRUCTURE CELLULAIRE; TEMPERATURA; TEMPERATURE; Thylakoids; TRANSPORT DES SUBSTANCES NUTRITIVES; TRANSPORTE DE NUTRIENTES; Cell compartment; Plant leaf; Spinacia oleracea; Nicotiana tabacum; Glycine max; Chenopodiaceae; Leguminosae; Dicotyledones; Pisum sativum; Angiospermae; Plant tissue disc; Plasma membrane; Cooling treatment; Spermatophyta; Solanaceae; Kinetics; Biological accumulation
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.97.4.1558

Leaf discs of four dicotyledonous species, when incubated at temperatures of 4 to 18 degrees C (optimum at 12 degrees C) for 30 or 60 minutes, responded by accumulations of membranes in the chloroplast stroma in the space between the inner membrane of the envelope and the thylakoids. The accumulated membranes, here referred to as the low temperature compartment, were frequently continuous with the envelope membrane and exhibited kinetics of formation consistent with a derivation from the envelope. Results were similar for expanding leaves of garden pea (Pisum sativum), soybean (Glycine max), spinach (Spinacia oleracea), and tobacco (Nicotiana tabacum). We suggest that the stromal low temperature compartment may be analogous to the compartment induced to form between the transitional endoplasmic reticulum and the Golgi apparatus at low temperatures. The findings provide evidence for the possibility of a vesicular transfer of membrane constituents between the inner membrane of the chloroplast envelope and the thylakoids of mature chloroplasts in expanding leaves