A study of the effects of therapeutic doses of ionizing radiation in vitro on Lactobacillus isolates originating from the vagina - a pilot study

• Published in: BMC microbiology Vol. 16; no. 1; p. 99
• Main Authors: Gosiewski, Tomasz, Mróz, Tomasz, Ochońska, Dorota, Pabian, Wojciech, Bulanda, Malgorzata, Brzychczy-Wloch, Monika
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 31-05-2016; BioMed Central
• Subjects: Bacteria; Cancer therapies; Dose-Response Relationship, Radiation; Female; Gamma rays; Gastrointestinal Tract - microbiology; Health aspects; Healthy Volunteers; Humans; Ionizing radiation; Irritable bowel syndrome; Lactobacillus; Lactobacillus - classification; Lactobacillus - isolation & purification; Lactobacillus - radiation effects; Microbiota; Pelvis; Physiological aspects; Pilot Projects; Probiotics; Quality of life; Radiation Dosage; Radiation therapy; Radiation Tolerance; Radiation, Ionizing; Vagina; Vagina - microbiology; Womens health; X-rays; United States; Ionizing radiation; Microbial flora; Lactobacillus
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-016-0716-5

Ionizing radiation is used as a therapeutic option in the treatment of certain neoplastic lesions located, among others, in the pelvic region. The therapeutic doses of radiation employed often result in adverse effects manifesting themselves primarily in the form of genital tract infections in patients or diarrhea. The data available in the literature indicate disorders in the microbial ecosystem caused by ionizing radiation, which leads to the problems mentioned above. In the present study, we examined the influence of ionizing radiation on 52 selected strains of bacteria: Lactobacillus crispatus, L. fermentum, L. plantarum, L. reuteri, L. acidophilus L. amylovorus, L. casei, L. helveticus, L. paracasei, L. rhamnosus, L. salivarius and L. gasseri. This collection of Lactobacillus bacteria isolates of various species, obtained from the genital tract and gastrointestinal tract of healthy women, was tested for resistance to therapeutic doses of ionizing radiation.
The species studied, were isolated from the genital tract (n = 30) and from the anus (n = 22) of healthy pregnant women. Three doses of 3 Gy (fractionated dose) and 50 Gy (total dose of the whole radiotherapy cycle) were applied. The greatest differences in survival of the tested strains in comparison to the control group (not subjected to radiation) were observed at the dose of 50 Gy. However, the results were not statistically significant. Survival decrease to zero was not demonstrated for any of the tested strains.
Therapeutic doses of radiation do not affect the Lactobacillus bacteria significantly.