The isolation and characterization of simultaneous saccharification and fermentation microorganisms for Laminaria japonica utilization

• Published in: Bioresource technology Vol. 102; no. 10; pp. 5962 - 5967
• Main Authors: Lee, Sung-Mok, Lee, Jae-Hwa
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-05-2011; Elsevier
• Subjects: Alginate lyase; Alginates; Bio-ethanol; Biofuel production; Biological and medical sciences; Biotechnology; Candida tropicalis; Carbohydrate Metabolism; Culture; Culture Media; Energy; Ethanol; Ethyl alcohol; Fermentation; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Laminaria - metabolism; Laminaria japonica; Mannitol; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microorganisms; Nuruk; Polysaccharide-Lyases - metabolism; Seaweeds; Alginate lyase; Nuruk; Laminaria japonica; Bio-ethanol; Fermentation; Ethanol; Enzyme; Algae; Saccharification; Marine alga; Biofuel; Lyases; Alginates; Edible alga; Heterokontophyta; Microorganism; Phaeophyceae
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2011.02.006

Brown seaweed contains various carbohydrates, such as alginate, laminaran, and mannitol, therefore ethanol fermentation was attempted with Nuruk and a mixed culture that included
Laminaria japonica. Nuruk is used to make Korean traditional alcohol. In the research, four microorganisms that produced ethanol and had the ability to achieve alginate degradation were obtained on the
L. japonica medium. Nuruk 4 was found to produce a better result than the other tested microorganisms, and the optimal substrate for ethanol production was found to be mannitol (2.59
g/L at 96
h). Nuruk 4 was more than three times better compared with
Candida tropicalis in regards to ethanol production. When alginate lyase activity occurred, it appeared as a clear zone around Nuruk 3. The maximal ethanol production yield conditions were comprised of Nuruk 3 and 4 on the anaerobic culture. In this case, 2.0
g/L of ethanol were efficiently produced under the same conditions.