Expression of Acidic and Basic Fibroblast Growth Factors in the Substantia Nigra of Rat, Monkey, and Human

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 88; no. 22; pp. 10237 - 10241
• Main Authors: Bean, Andrew J., Elde, Robert, Cao, Yihai, Oellig, Cornelia, Tamminga, Carol, Goldstein, Menek, Pettersson, Ralf F., Hokfelt, Tomas
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 15-11-1991; National Acad Sciences
• Subjects: Animals; Biochemistry and metabolism; Biological and medical sciences; Blotting, Northern; Brain; Central nervous system; Cerebellum; Cerebral Ventricles - drug effects; Cerebral Ventricles - physiology; Colchicine - pharmacology; Fibroblast Growth Factor 1 - genetics; Fibroblast Growth Factor 2 - genetics; Fibroblast growth factors; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Hydroxydopamines - pharmacology; Macaca fascicularis; Male; Mesencephalon; Messenger RNA; Neuroglia; Neurons; Neuroscience; Rats; Rats, Inbred Strains; RNA; RNA, Messenger - analysis; RNA, Messenger - genetics; Species Specificity; Substantia nigra; Substantia Nigra - cytology; Substantia Nigra - drug effects; Substantia Nigra - physiology; Vertebrates: nervous system and sense organs; Human; Fibroblast growth factor; Molecular hybridization; Rat; Immunocytochemistry; In situ; Rodentia; Monkey; Central nervous system; Vertebrata; Mammalia; Locus niger; Primates; Immunoblotting assay; Brain (vertebrata)
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.88.22.10237

The distribution of acidic (aFGF) and basic (bFGF) fibroblast growth factor mRNA and protein were examined in mesencephalon by immunohistochemistry, immunoblot analysis, in situ hybridization histochemistry, and RNA analysis. Coexistence of aFGF or bFGF with tyrosine hydroxylase protein in nigral cells was observed with immunohistochemistry. Both aFGF and bFGF mRNAs were found in the substantia nigra. Unilateral 6-hydroxydopamine lesions of nigrostriatal neurons resulted in a loss of aFGF and tyrosine hydroxylase [L-tyrosine, tetrahydropteridine: oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] mRNA-positive neurons on the lesioned side. The distribution of aFGF mRNA in monkey brain was similar to that seen in the rat. RNA and immunoblot analysis confirmed the presence of both aFGF and bFGF mRNAs and proteins in the substantia nigra of rat, monkey, and human.