Anti-inflammatory effects of chlorogenic acid from Taraxacum officinale on LTA-stimulated bovine mammary epithelial cells via the TLR2/NF-κB pathway

• Published in: PloS one Vol. 18; no. 3; p. e0282343
• Main Authors: Xu, Ping, Xu, Xiaobo, Fotina, Hanna, Fotina, Tetiana
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22-03-2023; Public Library of Science (PLoS)
• Subjects: Acids; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - pharmacology; Biology and Life Sciences; Cattle; Chlorogenic acid; Chlorogenic Acid - pharmacology; Chromatography; Dairy cattle; Dairy industry; Epithelial cells; Epithelial Cells - metabolism; Epithelium; Female; Gene expression; Gram-positive bacteria; High performance liquid chromatography; IL-1β; Infections; Inflammatory diseases; Interleukin 6; Interleukin-6 - genetics; Interleukin-6 - pharmacology; Kinases; Laboratories; Lipopolysaccharides - pharmacology; Lipoteichoic acid; Liquid chromatography; Mammary gland; Mammary Glands, Animal - metabolism; Mastitis; Medicine and Health Sciences; Microorganisms; Milk; NF-kappa B - metabolism; NF-κB protein; Pathways; Phenolic acids; Phenols; Phosphorylation; Proteins; Signal transduction; Signaling; Taraxacum; Taraxacum officinale; TLR2 protein; Toll-Like Receptor 2 - genetics; Toll-Like Receptor 2 - metabolism; Toll-like receptors; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; United States > US; China; Japan; Ukraine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0282343

Mastitis is an inflammatory disease caused by microbial infection. Chlorogenic acid (CGA), one of the major phenolic acids in Taraxacum officinale, has natural antioxidant and anti-inflammatory properties in various cell types; however, the effects of CGA on Lipoteichoic acid (LTA)-induced bovine mammary epithelial cells (BMECs) have not been investigated. In this study, the CGA content in T. officinale was determined by High-performance liquid chromatography (HPLC). BMECs were infected with LTA to induce the mastitis model. Different concentrations of CGA were administered after establishing the LTA infection. The results showed that the T. officinale contained CGA 1.36 mg/g. CGA significantly reduced the pro-inflammatory gene and protein expression of TNF-α, IL-6, and IL-1β. In addition, CGA downregulated the NO, TLR2, and NF-κB signaling pathways in LTA-infected bovine mammary epithelial cells. Our results indicate that CGA reduced the expression of TNF-α, IL-6, IL-1β, and TLR2 by inhibiting the phosphorylation of proteins in the NF-κB signaling pathways in a dose-dependent manner. This finding suggests that CGA may be a potential agent for the treatment of mastitis in dairy cows.