Antimicrobial susceptibility of Erysipelothrix rhusiopathiae isolated from pigs in southern Japan with a modified agar dilution method

• Published in: Journal of Veterinary Medical Science Vol. 72; no. 5; pp. 643 - 645
• Main Authors: Chuma, T., Kagoshima Univ. (Japan). Faculty of Agriculture, Kawamoto, T, Shahada, F, Fujimoto, H, Okamoto, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-05-2010; Japan Science and Technology Agency
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; antimicrobial resistance; ANTIMICROBIALS; ANTIMICROBIANOS; ANTIMICROBIEN; CERDO; DRUG RESISTANCE; ENSAYO; Erysipelothrix - classification; Erysipelothrix - drug effects; Erysipelothrix - isolation & purification; Erysipelothrix Infections - drug therapy; ERYSIPELOTHRIX RHUSIOPATHIAE; http://www.fao.org/aos/agrovoc#c_10471; http://www.fao.org/aos/agrovoc#c_35449; http://www.fao.org/aos/agrovoc#c_4788; http://www.fao.org/aos/agrovoc#c_509; http://www.fao.org/aos/agrovoc#c_7555; http://www.fao.org/aos/agrovoc#c_7683; Japan; METHODE; METHODS; METODOS; Microbial Sensitivity Tests; PORCIN; RESISTANCE AUX MEDICAMENTS; RESISTENCIA A MEDICAMENTOS; Species Specificity; SWINE; Swine - microbiology; Swine Diseases - drug therapy; Swine Diseases - microbiology; TESTAGE; TESTING; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.09-0448

The determination of antimicrobial minimum inhibitory concentration (MIC) in Erysipelothrix rhusiopathiae by using the agar dilution method has not been covered by the Clinical and Laboratory Institute (CLSI). Only the broth microdilution method has been outlined. This report describes a modification of the agar dilution procedure for E. rhusiopathiae using Trypto-soy agar supplemented with 0.1 % Tween 80 and incubation in ambient air at 37degC for 24 hr. The MICs of the assay were in agreement with those of the broth microdilution method recommended by the CLSI. Antimicrobial susceptibility test was performed using this method for 149 E. rhusiopathiae isolates from 2 meat processing plants in Kagoshima Prefecture during the period of April 2004 to March 2005. The number of strains resistant to oxytetracycline, erythromycin, lincomycin, ofloxacin and enrofloxacin were 56 (37.6%), 4 (2.7%),18 (12.1%), 21 (14.1%) and 19 (12.8%), respectively. All strains were susceptible to ampicillin.