Apelin, a New Enteric Peptide: Localization in the Gastrointestinal Tract, Ontogeny, and Stimulation of Gastric Cell Proliferation and of Cholecystokinin Secretion

• Published in: Endocrinology (Philadelphia) Vol. 145; no. 3; pp. 1342 - 1348
• Main Authors: Wang, Guiyun, Anini, Younes, Wei, Wei, Qi, Xiang, O’Carroll, Anne-Marie, Mochizuki, Tohru, Wang, Hui-Quin, Hellmich, Mark R, Englander, Ella W, Greeley, George H
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01-03-2004; Oxford University Press
• Subjects: Animals; Apelin; Biological and medical sciences; Calcium (intracellular); Calcium ions; Calcium Signaling - drug effects; Calcium signalling; Carrier Proteins - genetics; Carrier Proteins - metabolism; Carrier Proteins - pharmacology; Cell Division - drug effects; Cell Division - physiology; Cell growth; Cell Line; Cell proliferation; Cholecystokinin; Cholecystokinin - secretion; Epithelium; Female; Fetuses; Fundamental and applied biological sciences. Psychology; Gastrointestinal system; Gastrointestinal tract; Gene expression; Immunohistochemistry; In Vitro Techniques; Intercellular Signaling Peptides and Proteins; Intestine; Intracellular; Intracellular signalling; Kinases; Localization; Male; MAP kinase; Mitogen-Activated Protein Kinases - metabolism; mRNA; Ontogeny; Pancreas; Peptides; Pregnancy; Protein kinase C; Protein Kinase C - metabolism; Rats; Rats, Sprague-Dawley; RNA, Messenger - analysis; Stomach; Stomach - cytology; Stomach - metabolism; Vertebrates: endocrinology; Cell proliferation; Stomach; Peptides; Digestive system; Stimulation; Cholecystokinin; Gastrointestinal; Localization; Neuropeptide; Endocrine secretion
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2003-1116

Apelin is a recently discovered peptide that is the endogenous ligand for the APJ receptor. The aim of this study was to characterize apelin expression (mRNA levels) in the rat gastrointestinal tract and pancreas, to localize distribution of apelin peptide-containing cells in the stomach by immunohistochemistry, and to characterize the ontogeny of gastric apelin expression and peptide and the influence of apelin on gastric cell proliferation in vitro. Additionally, the effect of apelin on cholecystokinin (CCK) secretion and the involvement of MAPK, protein kinase C, and changes in intracellular Ca2+ in apelin-induced CCK secretion in vitro were examined. Northern analysis showed a maximal apelin expression in the stomach with a lower expression level in the intestine. Apelin expression was not detected in the pancreas. Immunohistochemistry revealed abundant apelin-positive cells in the glandular epithelium of the stomach. The ontogeny study showed a higher apelin expression in the fetal and postnatal rat stomachs when compared with the adult stomach. In contrast to apelin expression, apelin peptide was not detected in the rat stomach until 20 d of age and then increased progressively with age. Apelin was shown to stimulate gastric cell proliferation in vitro. Apelin also stimulated CCK secretion from a murine enteroendocrine cell line (STC-1); apelin-stimulated CCK secretion is mediated through MAPK but not by intracellular Ca2+ signaling. Together, these data indicate that apelin is an important new stomach peptide with a potential physiological role in the gastrointestinal tract.