Maturation-dependent changes of the rabbit reticulocyte energy metabolism

• Published in: FEBS letters Vol. 250; no. 1; pp. 40 - 44
• Main Authors: Kostić, M.M., Rapoport, S.M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 19-06-1989; Elsevier
• Subjects: 2,3-Diphosphoglycerate; Adenine nucleotide; Adenine Nucleotides - blood; Adenosine Diphosphate - blood; Adenosine Triphosphate - blood; Animals; Biological and medical sciences; Biphosphoglycerate, 2,3; Cell structures and functions; cyclic AMP; Cyclic AMP - blood; Diphosphoglyceric Acids - blood; Energy Metabolism; Erythrocyte Aging; Fundamental and applied biological sciences. Psychology; Glycolysis; Mitochondria and cell respiration; Molecular and cellular biology; Oxygen Consumption; Rabbits; Respiration; Reticulocyte; Reticulocytes - metabolism; Glycolysis; cyclic AMP; Reticulocyte; Respiration; Adenine nucleotide; Biphosphoglycerate, 2,3; Energy metabolism; Cell respiration
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(89)80680-5

Rabbit reticulocytes were separated into four fractions of different maturity in order to investigate the changes of cellular respiration and glycolysis, adenine nucleotides, 2,3-biphosphoglycerate (2,3-BPG) as well as cyclic AMP level during the transition from the youngest to the most mature reticulocytes. A significant reduction of total oxygen consumption, mainly due to depression of coupled respiration was found. The decline of respiration was accompanied by a 2-fold increase of the rate of aerobic glycolysis indicating a reduced Pasteur effect during maturation. A decline of ATP and an increase of ADP concentration was found. The oxygen-delivery capacity of the red cells increased by about 26% caused by an increase of the 2,3-BPG level of about 2 mmol/l cells. Cyclic AMP level in the fraction of youngest reticulocytes was about 60-fold higher than that in mature rabbit erythrocytes. The biggest decline of cyclic AMP was registered during the transition from youngest to the intermediate stage of maturity.