Interaction of sulforaphane with DNA and RNA

• Published in: PloS one Vol. 10; no. 6; p. e0127541
• Main Authors: Abassi Joozdani, Farzaneh, Yari, Faramarz, Abassi Joozdani, Parvaneh, Nafisi, Shohreh
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01-06-2015; Public Library of Science (PLoS)
• Subjects: Anticancer properties; Antioxidants; Binding; Binding sites; Biology; Cancer; Cell cycle; Conformation; Deoxyribonucleic acid; DNA; DNA - chemistry; Drugs; Enzymes; Fourier transforms; Gene expression; Grooves; Inflammation; Isothiocyanate; Isothiocyanates - chemistry; Nucleic Acid Conformation; Phosphates; Polyamines; Proteins; Ratios; Ribonucleic acid; RNA; RNA - chemistry; Sodium; Spectroscopy, Fourier Transform Infrared; Sulforaphane; Sulfoxides; Vegetables; Iran; United States > US; California
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0127541

Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables with anti-inflammatory, anti-oxidant and anti-cancer activities. However, the antioxidant and anticancer mechanism of sulforaphane is not well understood. In the present research, we reported binding modes, binding constants and stability of SFN-DNA and -RNA complexes by Fourier transform infrared (FTIR) and UV-Visible spectroscopic methods. Spectroscopic evidence showed DNA intercalation with some degree of groove binding. SFN binds minor and major grooves of DNA and backbone phosphate (PO2), while RNA binding is through G, U, A bases with some degree of SFN-phosphate (PO2) interaction. Overall binding constants were estimated to be K(SFN-DNA)=3.01 (± 0.035)×10(4) M(-1) and K(SFN-RNA)= 6.63 (±0.042)×10(3) M(-1). At high SFN concentration (SFN/RNA = 1/1), DNA conformation changed from B to A occurred, while RNA remained in A-family structure.