Characterization of HIV-1 genetic diversity and antiretroviral resistance in the state of Maranhão, Northeast Brazil

• Published in: PloS one Vol. 15; no. 3; p. e0230878
• Main Authors: Leal, Élcio, Arrais, Claudia Regina, Barreiros, Marta, Farias Rodrigues, Jessyca Kalynne, Silva Sousa, Nilviane Pires, Duarte Costa, Daniel, Rodrigo Pereira Santos, Francisco Dimitre, Dantas Silva, Antonio, Silva Viana, Antonia Iracilda E, Barros, Allan Kardec, Lima, Kledoaldo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 2020; Public Library of Science (PLoS)
• Subjects: Acquired immune deficiency syndrome; Adequacy; AIDS; Antiretroviral agents; Antiretroviral drugs; Antiretroviral therapy; Biology and Life Sciences; Biotechnology; Clusters; Computer and Information Sciences; Disease control; Drug resistance; Drug therapy; Electrical engineering; Epidemics; Epidemiology; Gene sequencing; Genetic diversity; Hepatitis; HIV; Human immunodeficiency virus; Infections; Information systems; Laboratories; Medicine and Health Sciences; Mutation; Nucleoside analogs; Nucleosides; People and places; Phylogenetics; Phylogeny; Pol gene; Prevention; Protease inhibitors; Proteinase inhibitors; Recombination; Research and Analysis Methods; Sexually transmitted diseases; STD; Surveillance; Brazil; United States > US; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0230878

The HIV-1 epidemic in Brazil has been growing in northeast and north regions, particularly an increase in AIDS cases among the younger male population has been observed. This study aims to characterize the HIV-1 genetic diversity and to evaluate its antiretroviral resistance profile among individuals presenting virological failure in the state of Maranhão-Brazil. HIV-1 pol gene sequences from 633 patients on antiretroviral therapy were obtained from the Department of Surveillance, Prevention and Control of Sexually Transmitted Infections, HIV/AIDS and Viral Hepatitis of the Brazilian Ministry of Health. Phylogenetic and recombination analyses were performed to characterize viral genetic diversity. The presence of antiretroviral resistance mutations was assessed using the HIV Drug Resistance Database online platform of Stanford University. A predominance of subtype B (84.5%) was observed, followed by recombinant BF (9.5%), where more than half of the sequences were dispersed in 3 clusters. Antiretroviral resistance was detected in 74.1% of the sequences, and it was significantly higher for nucleoside analogue reverse-transcriptase inhibitors (NRTIs) than for non-nucleoside analogue reverse-transcriptase inhibitors (NNRTIs) and protease inhibitors (PIs). Inference of putative transmissions clusters identified 11 clusters with 22 query sequences (22/633, 3.5%). Thus, we conclude that continuous monitoring of the molecular epidemiology of HIV-1 is essential for prevention strategies, epidemic control, and treatment adequacy.