A CAPS marker linked to a genic male-sterile gene in the colored sweet pepper, 'Paprika' (Capsicum annuum L.)

• Published in: Breeding Science Vol. 60; no. 1; pp. 93 - 98
• Main Authors: Lee, J., Seoul National Univ. (Korea R.), Han, J.H, An, C.G, Lee, W.P, Yoon, J.B
• Format: Journal Article
• Language: English
• Published: Tokyo Japanese Society of Breeding 2010; Japan Science and Technology Agency
• Subjects: AFLP; CAPS; CAPSICUM ANNUUM; ESTERILIDAD MASCULINA; GENE; GENES; GENETIC MARKERS; genic male sterility; GMS; http://www.fao.org/aos/agrovoc#c_1288; http://www.fao.org/aos/agrovoc#c_24030; http://www.fao.org/aos/agrovoc#c_3214; http://www.fao.org/aos/agrovoc#c_4537; INFERTILITE MALE; MALE INFERTILITY; MARCADORES GENETICOS; MARQUEUR GENETIQUE; paprika; pepper
• ISSN: 1344-7610; 1347-3735
• DOI: 10.1270/jsbbs.60.93

Genic male sterility (GMS) has been widely used as a tool for hybrid seed production in chili pepper (Capsicum annuum L.). Little work has been done on the development of molecular markers linked to pepper GMS genes, which are generally controlled by a recessive nuclear gene. In this study, we developed a DNA marker linked to paprika GMS using bulked segregant analysis (BSA) and an amplified fragment length polymorphism (AFLP) technique. Two Fsub(2) populations were made by selfing commercial Fsub(1) varieties of 'Mirage'and 'Fiesta'and subjected to BSA-AFLP using 256 primer combinations. Among five reproducible polymorphic primer combinations, an AFLP marker Egat/Mcgg was converted to a codominant cleavage amplified polymorphic sequence (CAPS) marker. This marker, named PmsM1-CAPS, is located about 2 to 3 cM from the ms locus. Although PmsM1-CAPS was not correlated with GMS in 'MiniBell'because it was a different GMS gene, the marker was found to be useful in screening for male sterility, as tested in Fsub(2) progenies from 'Helsinki'and Fsub(3) families derived from the Fsub(1) varieties used in this study.