Infection of Newborn Piglets with Bordetella pertussis: a New Model for Pertussis

• Published in: Infection and Immunity Vol. 73; no. 6; pp. 3636 - 3645
• Main Authors: Elahi, S, Brownlie, R, Korzeniowski, J, Buchanan, R, O'Connor, B, Peppler, M. S, Halperin, S. A, Lee, S. F, Babiuk, L. A, Gerdts, V
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-06-2005
• Subjects: Animals; Animals, Newborn; Bacterial diseases; Bacterial Infections; Bacteriology; Biological and medical sciences; Bordetella pertussis; CHO Cells; Colony Count, Microbial; Cricetinae; Disease Models, Animal; Ent and stomatologic bacterial diseases; Female; Fundamental and applied biological sciences. Psychology; Human bacterial diseases; Infectious diseases; Lung - microbiology; Lung - pathology; Medical sciences; Microbiology; Miscellaneous; Pertussis Vaccine - immunology; Swine; Vaccination; Whooping Cough - immunology; Whooping Cough - microbiology; Whooping Cough - pathology; Human; Infection; Newborn; Microbiology; Respiratory disease; Bacteriosis; Bacteria; Models; Bordetella pertussis; Immunity; Whooping cough
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.73.6.3636-3645.2005

Bordetella pertussis is the causative agent of pertussis or whooping cough. This bacterium is a human pathogen that under experimental conditions also infects selected rodents and primates. Here, we show for the first time that newborn piglets can be infected with B. pertussis when it is delivered intrapulmonarily. Infected piglets displayed fever and respiratory symptoms, such as nasal discharge, nonparoxysmal coughing, and breathing difficulties. Eventually, all infected animals developed severe bronchopneumonia, which in some cases was combined with a fibrinous pleuritits. Immunohistochemical staining revealed the presence of large numbers of B. pertussis cells within airways, adhering to the epithelial lining or phagocytosed by macrophages and neutrophils. Viable bacteria were reisolated from bronchoalveolar lavages and lung lesions for more than 10 days postinfection. The systemic presence of pertussis toxin was shown by hypoglycemia, lymphocytosis, and induction of a clustered pattern of CHO cells by serum and bronchoalveolar lavage samples. Thus, a large-animal model for pertussis was developed, which should complement existing rodent models for identifying the immune responses relevant to the design of new vaccines. In particular, this model should help researchers analyze the roles of both maternal and mucosal immunity in disease protection against pertussis and should ultimately assist in the design of new vaccines for early life protection.