Variations of gastric corpus microbiota are associated with early esophageal squamous cell carcinoma and squamous dysplasia

• Published in: Scientific reports Vol. 5; no. 1; p. 8820
• Main Authors: Nasrollahzadeh, Dariush, Malekzadeh, Reza, Ploner, Alexander, Shakeri, Ramin, Sotoudeh, Masoud, Fahimi, Saman, Nasseri-Moghaddam, Siavosh, Kamangar, Farin, Abnet, Christian C., Winckler, Björn, Islami, Farhad, Boffetta, Paolo, Brennan, Paul, Dawsey, Sanford M., Ye, Weimin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06-03-2015; Nature Publishing Group
• Subjects: 49; 49/23; 631/326/2565/2134; 631/67/1504/1477; Aged; Biodiversity; Carcinogenesis; Carcinoma, Squamous Cell - etiology; Carcinoma, Squamous Cell - pathology; Case-Control Studies; Deoxyribonucleic acid; DNA; Dysplasia; Esophageal cancer; Esophageal Neoplasms - etiology; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma; Esophagitis; Esophagus; Female; Gastric mucosa; Gastric Mucosa - microbiology; Gastric Mucosa - pathology; Humanities and Social Sciences; Humans; Male; Medicin och hälsovetenskap; Metagenome; Microbiota; Middle Aged; multidisciplinary; Neoplasm Staging; Observational studies; Precancerous Conditions; rRNA 16S; Science; Squamous cell carcinoma; Titanium
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep08820

Observational studies revealed a relationship between changes in gastric mucosa and risk of esophageal squamous cell carcinoma (ESCC) which suggested a possible role for gastric microbiota in ESCC carcinogenesis. In this study we aimed to compare pattern of gastric corpus microbiota in ESCC with normal esophagus. Cases were included subjects with early ESCC (stage I–II) and esophageal squamous dysplasia (ESD) as the cancer precursor. Control groups included age and sex-matched subjects with mid-esophagus esophagitis (diseased-control) and histologically normal esophagus (healthy-control). DNA was extracted from snap-frozen gastric corpus tissues and 16S rRNA was sequenced on GS-FLX Titanium. After noise removal, an average of 3004 reads per sample was obtained from 93 subjects. We applied principal coordinate analysis to ordinate distances from beta diversity data. Pattern of gastric microbiota using Unifrac (p = 0.004) and weighted Unifrac distances (p = 0.018) statistically varied between cases and healthy controls. Sequences were aligned to SILVA database and
Clostridiales
and
Erysipelotrichales
orders were more abundant among cases after controling for multiple testing (p = 0.011). No such difference was observed between mid-esophagitis and healthy controls. This study is the first to show that composition of gastric corpus mucosal microbiota differs in early ESCC and ESD from healthy esophagus.