Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS

• Published in: Molecules (Basel, Switzerland) Vol. 21; no. 11; p. 1471
• Main Authors: Liu, Liangliang, Leng, Juan, Yang, Xiai, Liao, Liping, Cen, Yin, Xiao, Aiping, Ma, Lei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05-11-2016; MDPI
• Subjects: 1-Butanol - chemistry; 1-Butanol - isolation & purification; bovine serum albumin; Chromatography; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - isolation & purification; Glucosides - chemistry; Glucosides - isolation & purification; Herbal medicine; Herbs; HPLC-MS; Isoflavones - chemistry; Isoflavones - isolation & purification; Ligands; magnetic nanoparticles; Magnetite Nanoparticles - chemistry; Mass Spectrometry; Medicine; Nanoparticles; Proteins; Radix astragali; Scientific imaging; Serum Albumin, Bovine - chemistry; China; ligands; Radix astragali; magnetic nanoparticles; HPLC-MS; bovine serum albumin
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules21111471

is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the
-butanol part of a
extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the
-butanol part of a
extract and identified as genistin (
), calycosin-7-
-β-d-glucoside (
), ononin (
) and formononetin (
). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation.